Cardiotrophin-1/CT-1 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Cardiotrophin-1/CT-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse Cardiotrophin‑1/CT‑1
  Ser2-Ala203
  Accession # Q60753
• Specificity: Detects mouse Cardiotrophin‑1/CT‑1 in ELISAs and Western blots. In sandwich immunoassays, less than 15% cross‑reactivity with recombinant human CT-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Cardiotrophin-1/CT-1 Antibody (Catalog # MAB438)
  ELISA Detection: Mouse Cardiotrophin-1/CT-1 Biotinylated Antibody (Catalog # BAF438)
  Standard: Recombinant Mouse Cardiotrophin-1/CT-1 (Catalog # 438-CT)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cardiotrophin-1/CT-1 Antibody [Biotin]

• cardiophin 1
• cardiotrophin 1
• Cardiotrophin1
• Cardiotrophin-1
• CT-1CT1
• CTF1

Background

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Mouse CT-1 encodes a 203 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has also been implicated in the receptor complex.

1. Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
2. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cardiotrophin-1/CT-1 Antibody (BAF438)(1)

Publication using BAF438

• Lesbordes JC, Bordet T, Haase G, Castelnau-Ptakhine L, Rouhani S, Gilgenkrantz H, Kahn A In vivo electrotransfer of the cardiotrophin-1 gene into skeletal muscle slows down progression of motor neuron degeneration in pmn mice. Hum. Mol. Genet., 2002-07-01;11(14):1615-25. 2002-07-01 [PMID: 12075006] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CTF1
• Uniprot:
  • Q60753()