Cardiotrophin-1/CT-1 Antibody

Recombinant Mouse Cardiotrophin‑1/CT‑1 (Catalog # 438-CT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Cardiotrophin‑1/CT‑1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Cardiotrophin‑1/CT‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF438). The ND50 is typically 0.08‑0.48 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cardiotrophin-1/CT-1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Cardiotrophin-1/CT‑1
  Ser2-Ala203
  Accession # Q60753
• Specificity: Detects Cardiotrophin‑1/CT‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross-reactivity with recombinant human CT-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTF1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.08-0.48 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cardiotrophin-1/CT-1 Antibody

• cardiophin 1
• cardiotrophin 1
• Cardiotrophin1
• Cardiotrophin-1
• CT-1CT1
• CTF1

Background

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Mouse CT-1 encodes a 203 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has also been implicated in the receptor complex.

1. Pennica, D. et al. (1996) Cytokine and Growth Factor Reviews 7:81.
2. Robledo, O. et al. (1997) J. Biol. Chem. 272:4855.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cardiotrophin-1/CT-1 Antibody (AF438)(2)

Publications using AF438

• Iniguez M, Berasain C, Martinez-Anso E, Bustos M, Fortes P, Pennica D, Avila MA, Prieto J Cardiotrophin-1 defends the liver against ischemia-reperfusion injury and mediates the protective effect of ischemic preconditioning. J. Exp. Med., 2006-12-18;203(13):2809-15. 2006-12-18 [PMID: 17178916] (Neutralization, Mouse)
• Lesbordes JC, Bordet T, Haase G, Castelnau-Ptakhine L, Rouhani S, Gilgenkrantz H, Kahn A In vivo electrotransfer of the cardiotrophin-1 gene into skeletal muscle slows down progression of motor neuron degeneration in pmn mice. Hum. Mol. Genet., 2002-07-01;11(14):1615-25. 2002-07-01 [PMID: 12075006] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: CTF1
• Uniprot:
  • Q60753()