CARD6 Antibody - BSA Free

Immunohistochemistry: CARD6 Antibody [NBP2-15704] - Induced expression of NOD2, CARD6, PYCARD and IFI16 in psoriasis lesions. Immunohistochemistry shows stronger NOD2, CARD6, PYCARD and IFI16 staining in PL (upper) than in controls (lower). PN (middle) and C are almost negative for NOD2 and CARD6. IFI16 is predominantly in nuclei of PL and PN. In controls IFI16 expression is weak and cytoplasmic. PYCARD is strongly induced in cytoplasm but also some nuclei are positive in PL and PN. Controls exhibit only a few PYCARD positive nuclei. Scale bar 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep22745) licensed under a CC-BY license.

Western Blot: CARD6 Antibody [NBP2-15704] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: CARD6 Antibody [NBP2-15704] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: CARD6 Antibody [NBP2-15704] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.

Induced expression of NOD2, CARD6, PYCARD, & IFI16 in psoriasis lesions.(a) Immunohistochemistry shows stronger NOD2, CARD6, PYCARD, & IFI16 staining in PL (upper) than in controls (lower). PN (middle) & C are almost negative for NOD2 & CARD6. IFI16 is predominantly in nuclei of PL & PN. In controls IFI16 expression is weak & cytoplasmic. PYCARD is strongly induced in cytoplasm but also some nuclei are positive in PL & PN. Controls exhibit only a few PYCARD positive nuclei. Scale bar 50 μm. (b) IEM of PL shows PYCARD clusters in cytoplasm. (c) Immunofluorescence of keratinocytes colocalizes CARD6 with a mitochondrial MTCO2. (d) IEM of psoriatioc lesional samples also localized CARD6 in the mitochondria (arrow head). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26976200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CARD6 Antibody [NBP2-15704] - Induced expression of NOD2, CARD6, PYCARD, & IFI16 in psoriasis lesions.(a) Immunohistochemistry shows stronger NOD2, CARD6, PYCARD, & IFI16 staining in PL (upper) than in controls (lower). PN (middle) & C are almost negative for NOD2 & CARD6. IFI16 is predominantly in nuclei of PL & PN. In controls IFI16 expression is weak & cytoplasmic. PYCARD is strongly induced in cytoplasm but also some nuclei are positive in PL & PN. Controls exhibit only a few PYCARD positive nuclei. Scale bar 50 μm. (b) IEM of PL shows PYCARD clusters in cytoplasm. (c) Immunofluorescence of keratinocytes colocalizes CARD6 with a mitochondrial MTCO2. (d) IEM of psoriatioc lesional samples also localized CARD6 in the mitochondria (arrow head). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26976200), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, MS
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CARD6 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CARD6 Antibody - BSA Free (NBP2-15704) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-CARD6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CARD6. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARD6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Application Notes: Electron microscopy usage reported in scientific literature (PMID: 26976200).
• Theoretical MW: 116 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CARD6 Antibody - BSA Free

• CARD6
• caspase recruitment domain family, member 6
• caspase recruitment domain protein 6
• caspase recruitment domain-containing protein 6
• CINCIN1
• CINCIN1CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation

Background

This gene encodes a protein that contains a caspase recruitment domain (CARD), an antiparallel six-helical bundle that mediates homotypic protein-protein interactions. The encoded protein is a microtubule-associated protein that has been shown to interact with receptor-interacting protein kinases and positively modulate signal transduction pathways converging on activation of the inducible transcription factor NF-kB. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CARD6 Antibody (NBP2-15704)(1)

Publication using NBP2-15704

• Tervaniemi MH, Katayama S, Skoog T et al. NOD-like receptor signaling and inflammasome-related pathways are highlighted in psoriatic epidermis. Sci Rep. 2016-03-15 [PMID: 26976200] (IHC-P, ICC/IF, EM, Human)
  
  Details:
  HDAC1 antibody was used for WB analysis of abdominal aorta samples from control subjects and the patients undergoing abdominal aortic aneurysms /AAA.

Bioinformatics

• Gene Symbol: CARD6
• Uniprot:
  • Q9BX69(Human)