Carboxypeptidase B1/CPB1 Antibody

Images

 
Western Blot: Carboxypeptidase B1/CPB1 Antibody [NBP1-32337] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase B antibody diluted at 1:500.
Western Blot: Carboxypeptidase B Antibody [NBP1-32337] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:200.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Carboxypeptidase B1/CPB1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase B. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Dog (84%), Pig (81%), Bovine (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Carboxypeptidase B1/CPB1 Antibody

  • carboxypeptidase B
  • carboxypeptidase B1 (tissue)
  • Carboxypeptidase B1
  • CPB
  • CPB1
  • DKFZp779K1333
  • EC 3.4.17
  • EC 3.4.17.2
  • Pancreas-specific protein
  • PASP
  • PCPB
  • procarboxypeptidase B

Background

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Carboxypeptidase B1/CPB1 Antibody (NBP1-32337). (Showing 1 - 2 of 2 FAQ).

  1. I use porcine carboxypeptidase B (CPB) in my fermentation process. I want to detect the residual CPB in my finished product preferably by ELISA/western blot. How will antibody pairs from Novus Biologics help me do that? Is a colorimetric assay possible with the antibody pair?
    • Any detection system can be employed when using the antibody pairs. The detection system will depend on the secondary used against the detection primary antibody from the pair, and the conjugate on the secondary antibody. The pair will work effectively in capturing the protein of interest, and subsequently detecting it with the second antibody in the pair for ELISA. This pair is not evaluated in Western Blot directly, but there Novus carries similar primary antibodies that we have that are validated in that application.
  2. I am trying to establish ELISA detection for carboxy peptidase. I am using a rat recombinant carboxypeptodase b from Roche diagnostics (cat no.03358682103). Can you please suggest the suitable kit.
    • Here is a link to our CPB1 products. H00001360-AP21 and H00001360-AP11 may suit your needs.

Secondary Antibodies

 

Isotype Controls

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Diseases for Carboxypeptidase B1/CPB1 Antibody (NBP1-32337)

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Bioinformatics

Gene Symbol CPB1
Entrez