Carbonic Anhydrase XII/CA12 Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase XII/CA12 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XII/CA12
  Ala25-Ser301
  Accession # Q8CI85
• Specificity: Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human CA12 is observed and less than 5% cross-reactivity with recombinant human CA1, rhCA2, rhCA3, rhCA8, recombinant mouse (rm) CA4, and rmCA9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Car12
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase XII/CA12 Antibody [Unconjugated]

• CA12
• Carbonate dehydratase XII
• carbonic anhydrase 12
• Carbonic Anhydrase XII
• carbonic anhydrase XIICAXII
• carbonic dehydratase
• CA-XII
• EC 4.2.1.1
• FLJ20151
• HsT18816
• Tumor antigen HOM-RCC-3.1.3

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Mouse CA12 is predicted to be a type I membrane enzyme (2, 3). The secreted, purified rmCA12 corresponds to the extracellular domain and has both CA activity and esterase activity. To our knowledge, this is the first description of purified rmCA12 with enzymatic activity.

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
3. Okazaki, Y. et al. (2002) Nature 420:563.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbonic Anhydrase XII/CA12 Antibody (AF2345)(3)

Publications using AF2345

• Alexander Chamessian, Michael Young, Yawar Qadri, Temugin Berta, Ru-Rong Ji, Thomas Van de Ven Transcriptional Profiling of Somatostatin Interneurons in the Spinal Dorsal Horn Scientific Reports 2018-05-01 [PMID: 29717160]
• Akiba Y, Mizumori M, Kuo M, Ham M, Guth PH, Engel E, Kaunitz JD CO2 chemosensing in rat oesophagus. Gut, 2008-08-05;57(12):1654-64. 2008-08-05 [PMID: 18682519] (ICC, IHC-Fr, Rat)
• Akiba Y, Ghayouri S, Takeuchi T, Mizumori M, Guth PH, Engel E, Swenson ER, Kaunitz JD Carbonic anhydrases and mucosal vanilloid receptors help mediate the hyperemic response to luminal CO2 in rat duodenum. Gastroenterology, 2006-07-01;131(1):142-52. 2006-07-01 [PMID: 16831598] (IHC, Rat)

Bioinformatics

• Gene Symbol: Car12
• Uniprot:
  • Q8CI85()