Carbonic Anhydrase XII/CA12 Antibody (336103)

• Reactivity: Mu
• Applications: WB
• Clone: 336103
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase XII/CA12 Antibody (336103) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase XII/CA12
  Ala25-Ser301
  Accession # Q8CI85
• Specificity: Detects mouse Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In Western blots, 100% cross‑reactivity with recombinant human (rh) CA12, 10% cross-reactivity with rhCA2, and no cross-reactivity with rhCA1, 3, 5A, 5B, 6, 7, 8, 10, 11, 13, recombinant mouse CA4, 9, or 14 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Car12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase XII/CA12 Antibody (336103)

• CA12
• Carbonate dehydratase XII
• carbonic anhydrase 12
• Carbonic Anhydrase XII
• carbonic anhydrase XIICAXII
• carbonic dehydratase
• CA-XII
• EC 4.2.1.1
• FLJ20151
• HsT18816
• Tumor antigen HOM-RCC-3.1.3

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Mouse CA12 is a type I membrane enzyme with both CA activity and esterase activity (2, 3). The amino acid sequence of mouse CA12 shares 97%, 82%, and 68% identity with that of rat, human/chimpanzee/canine, and chicken.

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
3. Okazaki, Y. et al. (2002) Nature 420:563.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Car12
• Uniprot:
  • Q8CI85()