Carbonic Anhydrase XII/CA12 Antibody (315602) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 315602
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase XII/CA12 Antibody (315602) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase XII/CA12
  Ala25-Gln291
  Accession # O43570
• Specificity: Detects human Carbonic Anhydrase XII/CA12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, -2, -3, -4, -7, -8, -10, -11, -13 or -14 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CA12
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase XII/CA12 Antibody (315602) [Unconjugated]

• CA12
• Carbonate dehydratase XII
• carbonic anhydrase 12
• Carbonic Anhydrase XII
• carbonic anhydrase XIICAXII
• carbonic dehydratase
• CA-XII
• EC 4.2.1.1
• FLJ20151
• HsT18816
• Tumor antigen HOM-RCC-3.1.3

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA12 is a type I membrane enzyme highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis (2, 3). It is over-expressed in some renal cell cancers and in glaucoma (2, 4). Two alternatively spliced forms exist, which either contains or lacks a 11 amino acid segment just before the transmembrane domain (5). The secreted, purified recombinant human CA12 contains the residues that are common for both forms.

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Tuereci, O. et al. (1998) Proc. Natl. Acad. Sci. USA 95:7608.
3. Ivanov, S.V. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12596.
4. Liao, S.-Y. et al. (2003) J. Med. Genet. 40:257.
5. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbonic Anhydrase XII/CA12 Antibody (MAB2190)(1)

Publication using MAB2190

• MM Jørgensen, R Bæk, J Sloth, K Varming, OB Christians, NE Ditlevsen, N Rajaratnam Treatment with intravenous immunoglobulin increases the level of small EVs in plasma of pregnant women with recurrent pregnancy loss J. Reprod. Immunol., 2020-04-09;140(0):103128. 2020-04-09 [PMID: 32330775] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CA12
• Uniprot:
  • O43570()