Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488]

Flow Cytometry: Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488] [NBP2-89518AF488] - Human primary renal tubular epithelial cells stained with carbonic Anhydrase IV/CA4 antibody conjugated to Alexa Fluor 488. Cells were immediately fixed with 4% PFA after detachment from culture plates and prior to staining. Final Ab concentration was 1:50. Image from verified customer review.

• Reactivity: Hu
• Applications: ELISA, Flow, IHC
• Clone: 039
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 488

Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase IV/CA4 (Accession#: NP_000708.1; Met1-Lys283).
• Specificity: No cross-reactivity in ELISA with:
  
  Human CA2
  Human CA3
  Human CA5A
  Human CA5B
  Human CA8
  Human CA9
  Human CA10
  Human CA12
  Human CA13
  Human CA14
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CA4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined. Carbonic Anhydrase IV/CA4 Antibody validated for Flow Cytometry from a verified customer review.
  
  Recommended applications are based on validated applications from the unconjugated base product NBP2-89518. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488]

• CA4
• CAIV
• CA-IV
• Car4
• Carbonate dehydratase IV
• carbonic anhydrase 4
• Carbonic Anhydrase IV
• carbonic anhydrase IVRP17
• carbonic dehydratase IV
• EC 4.2.1.1
• retinitis pigmentosa 17 (autosomal dominant)
• RP17

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Carbonic Anhydrase IV/CA4 Antibody (NBP2-89518AF488) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Sean DeWolf Flow Human 10/31/2022

Summary

• Application: Flow Cytometry
• Sample Tested: Renal tubule
• Species: Human
• Lot: D110963

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: CA4