Carbonic Anhydrase III/CA3 Antibody (495018)

Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase III Monoclonal Antibody (Catalog # MAB2185) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase III at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 495018
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase III/CA3 Antibody (495018) Summary

• Immunogen: E. coli-derived recombinant human Carbonic Anhydrase III/CA3
  Ala2-Lys260
  Accession # P07451
• Specificity: Detects human Carbonic Anhydrase III/CA3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CA1, 2, 4, 5a, 5b, 6, 7, 8, or 9 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CA3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase III/CA3 Antibody (495018)

• CA3
• CAIII
• CA-III
• Car3
• Carbonate dehydratase III
• carbonic anhydrase 3
• Carbonic Anhydrase III
• carbonic anhydrase III, muscle specific
• EC 4.2.1.1

Background

Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA3 is an approximately 29 kDa cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2). It is present at high levels in skeletal muscle and much lower levels in other tissues, such as cardiac and smooth muscle. The amino acid sequence of human CA3 is 99%, 90% and 92% identical to that of chimpanzee, dog and mouse/rat.

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA3
• Uniprot:
  • P07451()