Carbonic Anhydrase I/CA1 Antibody [Unconjugated]

Western blot shows lysates of HEL 92.1.7 human erythroleukemic cell line, human liver tissue, and human colon tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Carbonic Anhydrase I/CA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HEL 92.1.7 human erythroleukemic cell line and human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 38 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Carbonic Anhydrase I/CA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carbonic Anhydrase I/CA1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Carbonic Anhydrase I/CA1
  Ala2-Phe261
  Accession # P00915
• Specificity: Detects human Carbonic Anhydrase I/CA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) CA2, rhCA3, rhCA4, rhCA8, rhCA9, rhCA10, and rhCA12 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CA1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase I/CA1 Antibody [Unconjugated]

• CA1
• CA-I
• Car1
• Carbonate dehydratase I
• carbonic anhydrase 1
• Carbonic anhydrase B
• Carbonic Anhydrase I
• carbonic anhydrase ICAB
• carbonic dehydratase
• EC 4.2.1.1

Background

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation (2). The activity of CA1 can also be measured by its ability to catalyze the reaction CO₂ + H₂O → HCO₃^- + H⁺, using a published method (3).

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.
3. Wilbur, K.M. and N.G. Anderson (1948) J. Biol. Chem. 176:147.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carbonic Anhydrase I/CA1 Antibody (AF2180)(2)

Publications using AF2180

• Rasool, SUA;Pandith, AA;Ganie, FA;Aein, QU;Manzoor, U;Afroze, D;Baba, AM;Rasool, Z;Dar, NA;Muzamil, S; IL-11-IL6ST-STAT3 signaling defines a convergent inflammatory axis in esophageal cancer subtypes Scientific reports 2026-04-05 [PMID: 41936612] (Immunohistochemistry, Western Blot, Human)
• Wei Wei, Yuezhang Wu, Dong-Dong Chen, Yuntao Song, Guohui Xu, Qi Shi et al. Proteomics profiling for the global and acetylated proteins of papillary thyroid cancers Proteome Science 2023-04-26 [PMID: 37101287]

Bioinformatics

• Gene Symbol: CA1
• Uniprot:
  • P00915()