CaMKIV Antibody

Western Blot: CaMKIV Antibody [NB120-3557] - Analysis of rat brain extract.

• Reactivity: Hu, Mu, Rt, Ca, Pl, Pm, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CaMKIV Antibody Summary

• Description: Novus Biologicals Rabbit CaMKIV Antibody (NB120-3557) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic Peptide: V(127) E K G Y Y S E R D A A D A V K Q(143) C
• Localization: Cytoplasmic and Nuclear
• Specificity: NB120-3557 detects CaM kinase IV (CaMKIV) from human, mouse, rat, canine, primate, arabidopsis and Xenopus Oocyte samples.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAMK4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1 ug/ml
  • Western Blot 0.5 ug/ml
• Application Notes: WB: Detects an approx. 55 kDa protein representing CaMKIV in HeLa cell lysate.
• Control:

  HeLa Whole Cell Lysate
  CaMKIV Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CaMKIV Antibody

• brain Ca(2+)-calmodulin-dependent protein kinase type IV
• brain Ca++-calmodulin-dependent protein kinase type IV
• calcium/calmodulin-dependent protein kinase IV
• calcium/calmodulin-dependent protein kinase type IV catalytic chain
• calcium/calmodulin-dependent protein kinase type IV
• CAM kinase- GR
• CAM kinase IV
• CaM kinase-GR
• CaMK IV
• caMK
• CaMK-GR
• EC 2.7.11
• EC 2.7.11.17
• IV
• MGC36771

Background

CaM kinase IV (CaMKIV), in a calcium and calmodulin dependent manner, is implicated in phosphorylating numerous transcription factors including the CCAAT enhancer binding protein (C / EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK4
• Entrez:
  • 814(Human)
  • 12326(Mouse)
  • 25050(Rat)