CaM Kinase I Antibody

Images

 
Western blot shows lysates of IMR‑32 human neuroblastoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human ...read more
CaM Kinase I was detected in immersion fixed paraffin-embedded sections of human hypothalamus using Sheep Anti-Human CaM Kinase I Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7899) at 1.7 µg/mL overnight ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CaM Kinase I Antibody Summary

Immunogen
E. coli-derived recombinant human CaM Kinase I alpha
Ser176-Arg254 (Ala224Ser)
Accession # Q14012
Specificity
Detects recombinant human CaM Kinase I alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CaMKIV is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CAMK1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CaM Kinase I Antibody

  • calcium/calmodulin-dependent protein kinase I
  • calcium/calmodulin-dependent protein kinase type 1
  • CaM kinase I alpha
  • CaM Kinase I
  • CAMKI
  • caM-KI
  • caMKI-alpha
  • EC 2.7.11
  • EC 2.7.11.17
  • MGC120317
  • MGC120318

Background

CaMKI (Calcium/Calmodulin-dependent kinase I) is a 37-43 kDa monomeric and cytosolic member of the CaMK subfamily, Ser/Thr protein kinase family, protein kinase superfamily of molecules.  It is found in multiple cell types, including adrenal cortical cells, neurons and macrophages/osteoclast precursors.  CaMKI is associated with the cellular Ca++ signaling pathway.  Upon entry into the cell, calcium levels are sensed by calmodulin.  Upon Ca++ binding, calmodulin (and CaMK kinase) interacts with a Ser/Thr protein kinase termed CaMKIa.  CaMKIa is of particular interest in neurons and cells of the zona glomerulosa.  In neurons, CaMKIa activation results in the recruitment of AMPA-Rs and the promotion of axon growth (vs.dendrite outgrowth which is promoted by CaMKIg). In the adrenal gland, CaMKIa regulates CYP11B2 transcription, and thus the ability to synthesize aldosterone.  Human CaMKI, the isoform used for immunization, is 370 amino acids (aa) in length (SwissProt #:Q14012).  It contains one protein kinase domain (aa 20-276) followed by a calmodulin-binding region (aa 296-317) and an NES (aa 315-321).   Over aa 178-257, human and mouse CaMKI are identical in amino acid sequence.  There are three additional CaMKI isoforms (b, g, d), all the product of distinct genes.  Nevertheless, over the same aa sequence, these three isoforms (b, g, d) share 80%, 81% and 97% aa sequence identity with CaMKI(a), respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CAMK1
Uniprot