Calretinin Antibody

Western Blot: Calretinin Antibody [NBP1-32244] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE Calretinin antibody, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Calretinin Antibody [NBP1-32244] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calretinin protein stained by Calretinin antibody diluted at 1:200. Blue: Hoechst 33342 staining.

Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen Section adult mouse hippocampus. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody [114] diluted at 1:500. Blue: Fluoroshield with DAPI.

Western Blot: Calretinin Antibody [NBP1-32244] - A. 30 ug 293T whole cell lysate/extract. B. 30 ug whole cell lysate/extract of human CALB2-transfected 293T cells.

Western Blot: Calretinin Antibody [NBP1-32244] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Calretinin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Calretinin Antibody [NBP1-32244] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244] - Mouse fore brain. Calretinin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Frozen: Calretinin Antibody [NBP1-32244] - Frozen sectioned adult mouse retina. Green: Calretinin protein stained by Calretinin antibody diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Calretinin Antibody [NBP1-32244] - Mouse retina, using Calretinin antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Calretinin Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.
• Marker: Mesothelial Cell Marker
• Predicted Species: Porcine (96%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CALB2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Calretinin Antibody

• 29 kDa calbindin
• CAB29
• CAL2
• CALB2
• calbindin 2
• calbindin 2, (29kD, calretinin)
• calbindin 2, 29kDa (calretinin)
• calbindin D29K
• Calretinin
• CR

Background

Calretinin (CAB29, calbindin 2) is a calcium binding protein that belongs to calbindin family. It is mainly found in neuronal tissue, high expressed in auditory neurons. It has been implicated in as a calcium buffer, calcium sensor, and as a regulator of apoptosis (1). Calretinin is considered as a specific marker of mesothelllal cells; it is the only marker that has the utility in distinguishing between sarcomatoid mesotheliomas and sarcomatoid renal cell carcinomas (2). It is widely used in the panel of makers for distinguishing mesothlioma from adenocarcinoma. Along with calretinin, the panel includes E-cadherin, CEA, and cytokeratin 5/6 (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Calretinin Antibody (NBP1-32244)(2)

Publications using NBP1-32244

• Brokhman I, Xu J, Coles BLK et al. Dual embryonic origin of the mammalian enteric nervous system. Dev. Biol. 2018-11-22 [PMID: 30472119] (ICC/IF, Mouse)
• Robinson CL, Chong ACN, Ashbrook AW et al. Male germ cells support long-term propagation of Zika virus Nat Commun 2018-05-29 [PMID: 29844387] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CALB2