Calreticulin Antibody (6C6)

Western Blot: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of NBP2-50053 in HEK293 (lane 1), 3T3 (lane 2), SHSY-5Y (lane 3) and HeLa cells (lane 4). NBP2-50053 binds strongly to Calreticulin in 50 kDa.

Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - Analysis of of SH-SY5Y cells stained with mouse mAb to calreticulin, NBP2-50053, dilution 1:500 in green and costained with chicken pAb to lamin A/C, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The MCA-6C6 antibody reveals granular staining of cytoplasm, while the lamin AC antibody stains the nuclear lamina and membrane.

Immunocytochemistry/Immunofluorescence: Calreticulin Antibody (6C6) [NBP2-50053] - HeLa cell cultures were stained with NBP2-50053 (red). Calreticulin predominately localized in vesicles and the ER. Cells were counterstained with chicken polyclonal antibody to Vimentin (NB300-223, green). Blue is a DNA stain.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 6C6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Calreticulin Antibody (6C6) Summary

• Immunogen: Synthetic peptide VESGSLEDDWDFLPPKKI corresponding to amino acids 191-208 of human Calreticulin, including the LC3 interacting region or LIR. [UniProt# P27797]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 - 1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:1000-1:5000
• Theoretical MW: 48 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Calreticulin Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50% PBS, 50% Glycerol
• Preservative: 5mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Calreticulin Antibody (6C6)

• Autoantigen Ro
• CALR
• Calregulin
• Calreticulin
• cC1qR
• CRP55
• CRT
• CRTC
• Endoplasmic reticulum resident protein 60
• ERp60
• FLJ26680
• grp60
• RO
• Sicca syndrome antigen A (autoantigen Ro; calreticulin)
• SSA
• SSAHACBP
• Vasostatin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALR
• Uniprot:
  • P27797(Human)