Calpain 10 Antibody

Images

 
Western Blot: Calpain 10 Antibody [H00011132-D01P] - Analysis of CAPN10 expression in transfected 293T cell line by CAPN10 polyclonal antibody.Lane 1: CAPN10 transfected lysate(74.90 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Calpain 10 Antibody [H00011132-D01P] - Analysis of antibody to CAPN10 on HeLa cell. Antibody concentration 30 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Calpain 10 Antibody Summary

Immunogen
CAPN10 (NP_075571.1, 1 a.a. ~ 672 a.a) full-length human protein.MRAGRGATPARELFRDAAFPAADSSLFCDLSTPLAQFREDITWRRPQEICATPRLFPDDPREGQVKQGLLGDCWFLCACAALQKSRHLLDQVIPPGQPSWADQEYRGSFTCRIWQFGRWVEVTTDDRLPCLAGRLCFSRCQREDVFWLPLLEKVYAKVHGSYEHLWAGQVADALVDLTGGLAERWNLKGVAGSGGQQDRPGRWEHRTCRQLLHLKDQCLISCCVLSPRAGARELGEFHAFIVSDLRELQGQAGQC
Specificity
Reacts with calpain 10.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPN10
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
This antibody is reactive against transfected lysate in western blot, and as a detection antibody in ELISA.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Calpain 10 Antibody

  • Calcium-activated neutral proteinase 10
  • calpain 10
  • calpain-10
  • calpain-like protease CAPN10
  • CANP 10
  • CANP10
  • EC 3.4.22.-
  • KIAA1845
  • NIDDM1

Background

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CAPN10
Entrez
Uniprot