Calpain 5 Antibody (HL2432) - Azide and BSA Free

Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Calpain 5 antibody [HL2432] detects Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Calpain 5 stained by Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Calpain 5 antibody [HL2432] detects secreted Calpain 5 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Calpain 5 stained by Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: Calpain 5 Antibody (HL2432) - Azide and BSA Free [NBP3-25394] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calpain 5 antibody [HL2432] (NBP3-25394) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: HL2432
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Calpain 5 Antibody (HL2432) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Calpain 5 Antibody (HL2432) - Azide and BSA Free (NBP3-25394) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Calpain 5.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAPN5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry Assay dependent
  • Immunohistochemistry-Paraffin Assay dependent
  • Western Blot 1:500-1:3000
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Calpain 5 Antibody (HL2432) - Azide and BSA Free

• calpain 5
• Calpain htra-3
• calpain-5
• EC 3.4.22
• EC 3.4.22.54
• FLJ46245
• HTRA3NCL3
• nCL-3EC 3.4.22.-
• New calpain 3

Background

Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAPN5