Calnexin Antibody

Images

 
Western Blot: Calnexin Antibody [NB100-1974] - Identification of plasma exosomes and differentially expressed exosomal miRNAs. Confirmation of the exosomes markers with Western blotting indicated the presence of CD63 ...read more
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1974] - Zebrafish Vwf forms pseudo-Weibel-Palade bodies (pseudo-WPBs) in mammalian cell culture. pzVwf/Myc-HIS (zebrafish Vwf) plasmids were transfected ...read more
Immunohistochemistry: Calnexin Antibody [NB100-1974] - Analysis of Calnexin in mouse bladder using DAB with hematoxylin counterstain.
Flow Cytometry: Calnexin Antibody [NB100-1974] - An intracellular stain was performed on HeLa cells with Calnexin antibody NB100-1974AF488 (blue) and a matched isotype control NBP2-24893AF488 (orange). Cells were fixed ...read more
Western Blot: Calnexin Antibody [NB100-1974] - Analysis of Calnexin in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: Calnexin Antibody [NB100-1974] - CANX antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Flow Cytometry: Calnexin Antibody [NB100-1974] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-1974. An intracellular stain was performed on Jurkat cells with Calnexin antibody NB100-1974AF488 (blue) and a matched ...read more
Simple Western: Calnexin Antibody [NB100-1974] - Image shows a specific band for Calnexin in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, Ca, Pm-OrSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Calnexin Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the rat Calnexin protein (between residues 550-591) [Uniprot: P35565]
Localization
Endoplasmic reticulum
Marker
Endoplasmic Reticulum Membrane Marker
Predicted Species
Canine (90%), Orangutan (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CANX
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:50
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:100
Application Notes
In ICC/IF, endoplasmic reticulum staining was observed in HeLa cells. In Western Blot, a band is seen at ~ 90 kDa representing Calnexin. In IHC-P, staining was observed in the endoplasmic reticulum of mouse bladder tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB100-1974 in the following applications:

Publications
Read Publications using
NB100-1974 in the following applications:

  • 1 publication
  • WB
    4 publications

Reactivity Notes

Hamster reactivity reported in the scientific literature (PMID: 23760268).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Calnexin Antibody

  • calnexin
  • CNX
  • IP90FLJ26570
  • Major histocompatibility complex class I antigen-binding protein p88
  • P90

Background

Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calnexin Antibody (NB100-1974)(5)

Review for Calnexin Antibody (NB100-1974) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NB100-1974:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
WB Other 12/12/2014
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23760268
SpeciesOther

Blocking

Blocking DetailsSee PMID 23760268

Primary Anitbody

Dilution Ratio1:1000 dilution

Details

Detection NotesSee PMID 23760268

Comments

CommentsPublished in PMID: 23760268

Product General Protocols

View specific protocols for Calnexin Antibody (NB100-1974): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calnexin Antibody (NB100-1974) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-1974AF350
Alexa Fluor 405 NB100-1974AF405
Alexa Fluor 488 NB100-1974AF488
Alexa Fluor 532 NB100-1974AF532
Alexa Fluor 594 NB100-1974AF594
Alexa Fluor 647 NB100-1974AF647
Alexa Fluor 700 NB100-1974AF700
Alexa Fluor 750 NB100-1974AF750
Biotin NB100-1974B
DyLight 350 NB100-1974UV
DyLight 405 NB100-1974V
DyLight 488 NB100-1974G
DyLight 550 NB100-1974R
DyLight 594 NB100-1974DL594
DyLight 650 NB100-1974C
DyLight 680 NB100-1974FR
DyLight 755 NB100-1974IR
FITC NB100-1974F
HRP NB100-1974H
Janelia Fluor 549 NB100-1974JF549
Janelia Fluor 646 NB100-1974JF646

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Bioinformatics Tool for Calnexin Antibody (NB100-1974)

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Blogs on Calnexin.

Calnexin - an ER chaperone that folds the cell's glycoproteins
Calnexin is an abundant 90kDa chaperone protein that resides in the membrane of the endoplasmic reticulum. Calnexin and the related calreticulin protein function together to ensure the proper folding of glycoproteins. By binding to partially folded...  Read full blog post.

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Recent Reviews

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4
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1
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Anonymous
12/12/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol CANX
Entrez
Uniprot