Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has ...read more
Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype ...read more
Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.
Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Simple Western lane view shows a specific band for Calcium Sensing Receptor in 0.5 mg/ml of Hek293 lysate. This experiment was performed under ...read more
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Tris-glycine, 150 mM NaCl
0.05% Sodium Azide
Protein G purified
Western Blot 1:50-1:200
Simple Western 1:200
Flow Cytometry 1 ug per million cells
Immunoprecipitation 5-10 ug
This Calcium Sensing Receptor antibody is useful for ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen sections, Immunoprecipitation, Radioimmunoassay and Western Blot. In Western blot transfected samples were tested, where a band was seen ~130 kDa and a large smear at the top of the gel.In Simple Western only 10-15 uL of the recommended dilution is used per data point. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-1830 in the following applications:
Alternate Names for Calcium-sensing R/CaSR Antibody (HL 1499)
extracellular calcium-sensing receptor
hypocalciuric hypercalcemia 1
parathyroid Ca(2+)-sensing receptor 1
Parathyroid cell calcium-sensing receptor
Calcium Sensing Receptor (CaSR) was the first GPCR identified whose main physiological ligand is an ion and it has the ability to sense extracellular concentration of Ca2+. CaSR's activity is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and it is capable of interaction with VCP, RNF19A (execute CASR's ubiquitination) and ARRB1. CaSR is expressed primarily in chief cells of parathyroid glands where, when activated by high Ca2+ concentrations, it decreases PTH release (calcium-retaining hormone) to maintain Ca2 +. Alternatively, under hypocalcemia conditions, CaSR is inactive and PTH is released which restores normocalcemia by acting on kidney as well as intestine to increase Ca2 + absorption, and on bone, to mobilise skeletal Ca2 +. CaSR is also expressed in other tissues which do not play an obvious role in Ca2 + homeostasis (breast, blood vessels, liver, and placenta) and altered CaSR expression and/or activity have been found associated with parathyroid glands disorders, osteoporosis, vascular calcification and cancer. CaSR is implicated in regulation of diverse processes that includes hormone secretion, gene expression, ion channel activity, modulation of inflammation, proliferation, differentiation, and apoptosis, depending on cell type, and therefore, represents a key molecule in physiology. Defects in CaSR have been linked to familial hypocalciuric hypercalcemia type 1 (FHH), neonatal severe primary hyperparathyroidism (NSHPT), familial isolated hypoparathyroidism (FIH) and epilepsy, idiopathic generalized type 8 (EIG8).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Szalat A, Benhamron S, Galitzer H et al. Treatment-Resistant Auto-Immune Hypoparathyroidism Associated with Celiac Disease. 90th Annual Meeting of the Endocrine Society (ENDO); San Francisco, CA; USA. 15-Jun-08.
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Product General Protocols
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