Calcium-sensing R/CaSR Antibody (HL 1499)

Images

 
Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has ...read more
Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx ...read more
Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype ...read more
Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.
Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Simple Western lane view shows a specific band for Calcium Sensing Receptor in 0.5 mg/ml of Hek293 lysate. This experiment was performed under ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, RbSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, RIA
Clone
HL 1499
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Calcium-sensing R/CaSR Antibody (HL 1499) Summary

Immunogen
N-terminal, extracellular region of human Calcium Sensing Receptor. [UniProt# P41180]
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASR
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:50-1:200
  • Simple Western 1:200
  • ELISA 1:100-1:2000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunoprecipitation 5-10 ug
  • Radioimmunoassay
Application Notes
This Calcium Sensing Receptor antibody is useful for ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry frozen sections, Immunoprecipitation, Radioimmunoassay and Western Blot. In Western blot transfected samples were tested, where a band was seen approx. 130 kDa and a large smear at the top of the gel.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Kidney Lysate (NB820-59231)
Calcium-sensing R/CaSR Lysate (NBL1-08721)
Publications
Read Publications using
NB100-1830 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Calcium-sensing R/CaSR Antibody (HL 1499)

  • calcium-sensing receptor
  • CAR
  • CaSR
  • EIG8
  • extracellular calcium-sensing receptor
  • FHH
  • FIH
  • GPRC2A
  • GPRC2AHHC
  • HHC1
  • hypocalciuric hypercalcemia 1
  • NSHPT
  • parathyroid Ca(2+)-sensing receptor 1
  • Parathyroid cell calcium-sensing receptor
  • PCaR1
  • PCAR1MGC138441

Background

Calcium Sensing Receptor (CaSR) was the first GPCR identified whose main physiological ligand is an ion and it has the ability to sense extracellular concentration of Ca2+. CaSR's activity is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and it is capable of interaction with VCP, RNF19A (execute CASR's ubiquitination) and ARRB1. CaSR is expressed primarily in chief cells of parathyroid glands where, when activated by high Ca2+ concentrations, it decreases PTH release (calcium-retaining hormone) to maintain Ca2 +. Alternatively, under hypocalcemia conditions, CaSR is inactive and PTH is released which restores normocalcemia by acting on kidney as well as intestine to increase Ca2 + absorption, and on bone, to mobilise skeletal Ca2 +. CaSR is also expressed in other tissues which do not play an obvious role in Ca2 + homeostasis (breast, blood vessels, liver, and placenta) and altered CaSR expression and/or activity have been found associated with parathyroid glands disorders, osteoporosis, vascular calcification and cancer. CaSR is implicated in regulation of diverse processes that includes hormone secretion, gene expression, ion channel activity, modulation of inflammation, proliferation, differentiation, and apoptosis, depending on cell type, and therefore, represents a key molecule in physiology. Defects in CaSR have been linked to familial hypocalciuric hypercalcemia type 1 (FHH), neonatal severe primary hyperparathyroidism (NSHPT), familial isolated hypoparathyroidism (FIH) and epilepsy, idiopathic generalized type 8 (EIG8).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calcium-sensing R/CaSR Antibody (NB100-1830)(4)

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Product General Protocols

View specific protocols for Calcium-sensing R/CaSR Antibody (NB100-1830): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calcium-sensing R/CaSR Antibody (NB100-1830) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-1830AF405
Alexa Fluor 488 Labeled NB100-1830AF488
Alexa Fluor 647 Labeled NB100-1830AF647
Alexa Fluor 700 Labeled NB100-1830AF700
Allophycocyanin Labeled NB100-1830APC
Biotin Labeled NB100-1830B
DyLight 350 Labeled NB100-1830UV
DyLight 405 Labeled NB100-1830V
DyLight 488 Labeled NB100-1830G
DyLight 550 Labeled NB100-1830R
DyLight 650 Labeled NB100-1830C
DyLight 680 Labeled NB100-1830FR
DyLight 755 Labeled NB100-1830IR
FITC Labeled NB100-1830F
HRP Labeled NB100-1830H
PE Labeled NB100-1830PE

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Bioinformatics

Gene Symbol CASR
Entrez