Cadherin-17 Antibody (PD00-49)

Western Blot: Cadherin-17 Antibody (PD00-49) [NBP3-32534] - Western blot analysis of Cadherin-17 on different lysates with Rabbit anti-Cadherin-17 antibody (NBP3-32534) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: LoVo cell lysate Lane 3: Human kidney tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 92 kDa Observed band size: 120 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32534) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Cadherin-17 Antibody (PD00-49) [NBP3-32534] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Rabbit anti-Cadherin-17 antibody (NBP3-32534) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32534) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry: Cadherin-17 Antibody (PD00-49) [NBP3-32534] - Immunofluorescence analysis of paraffin-embedded human colon tissue labeling Cadherin-17 with Rabbit anti-Cadherin-17 antibody (NBP3-32534) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32534, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

• Reactivity: Hu
• Applications: WB, IHC
• Clone: PD00-49
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Cadherin-17 Antibody (PD00-49) Summary

• Description: Novus Biologicals Rabbit Cadherin-17 Antibody (PD00-49) (NBP3-32534) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide. (Uniprot: Q12864)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDH17
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:200
  • Western Blot 1:1000
• Theoretical MW: 92 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cadherin-17 Antibody (PD00-49)

• cadherin 17, LI cadherin (liver-intestine)
• cadherin
• cadherin-16
• Cadherin17
• Cadherin-17
• CDH16
• CDH17
• FLJ26931
• HPT-1 cadherin
• HPT1
• HPT-1
• human intestinal peptide-associated transporter HPT-1
• human peptide transporter 1
• Intestinal peptide-associated transporter HPT-1
• LI cadherin
• LI-cadherin
• Liver-intestine cadherin
• MGC138218
• MGC142024

Background

LI Cadherin is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH17