Cadherin-16 Antibody (CDH16/1071)

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/1071) [NBP2-45157] - Human Renal Cell Carcinoma stained with KSP-Cadherin Monoclonal Antibody (CDH16/1071)

Flow Cytometry: Cadherin-16 Antibody (CDH16/1071) [NBP2-45157] - Analysis of human HEK293 cells usingCadherin-16 Antibody (CDH16/1071) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/1071) [NBP2-45157] - Mouse Kidney stained with Cadherin-16 Antibody (CDH16/1071).

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/1071) [NBP2-45157] - Human Kidney stained with Cadherin-16 Antibody (CDH16/1071).

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/1071) [NBP2-45157] - Human Kidney stained with Cadherin-16 Antibody (CDH16/1071) followed by Goat anti-Mouse IgG-CF640R (Purple)

Immunohistochemistry-Paraffin: Cadherin-16 Antibody (CDH16/1071) [NBP2-45157] - Rat Kidney stained with KSP-Cadherin Monoclonal Antibody (CDH16/1071)

Western Blot: Cadherin-16 Antibody (CDH16/1071) [NBP2-45157] - Analysis of human kidney tissue lysate usingCadherin-16 Antibody (CDH16/1071).

• Reactivity: Hu, Mu, Rt, Ca, Rb
• Applications: WB, ELISA, Flow, IHC
• Clone: CDH16/1071
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cadherin-16 Antibody (CDH16/1071) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47745)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant full-length human Cadherin-16 protein (Uniprot: O75309)
• Localization: Cell Surface with some cytoplasmic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH16
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-4 ug/ml
  • Flow Cytometry 1-2 ug/million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cadherin-16 Antibody (CDH16/1071)

• cadherin 16, KSP-cadherin
• cadherin-16
• Kidney-specific cadherin
• KSP-cadherin

Background

Cadherin 16 is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cadherin-16 Antibody (NBP2-45157)(3)

Publications using NBP2-45157

• Belghasem M, Yin W, Lotfollahzadeh S et al. Tryptophan Metabolites Target Transmembrane and Immunoglobulin Domain-Containing 1 Signaling to Augment Renal Tubular Injury The American journal of pathology 2023-10-01 [PMID: 37676196] (WB, Mouse)
• Li H, Tsokos MG, Bhargava R et al. Interleukin-23 reshapes kidney resident cell metabolism and promotes local kidney inflammation The Journal of clinical investigation 2021-05-06 [PMID: 33956666]
• Li ZH, An N, Huang XJ et al. Cyclosporine A blocks autophagic flux in tubular epithelial cells by impairing TFEB-mediated lysosomal function J Cell Mol Med 2021-05-05 [PMID: 33949118]
  
  Details:
  Citation using the Azide and BSA Free format of this antibody.

FAQs for Cadherin-16 Antibody (NBP2-45157). (Showing 1 - 1 of 1 FAQs).

1. I would like to know whether the following antibody can be used for flow cytometric analysis using living, non-permeablized cells.
   • Since the immunogen for raising this antibody was recombinant human CDH16 protein (full-length) and we don't map the epitopes of any antibody, we don't know where the antibody binds to in the human CDH16. This protein is a transmembrane protein. If wanting to use with the living cells with NBP2-45157, the epitope has to be located in the extracellular cell surface. Furthermore, this antibody contains 0.05% Sodium Azide, which is a toxin to the life cells. Therefore we would not recommend you to use NBP2-45157 with any living cells.

Bioinformatics

• Gene Symbol: CDH16
• Uniprot:
  • O75309(Human)