Cadherin-11 Antibody (HL1044) - Azide and BSA Free

Western Blot: Cadherin-11 Antibody (HL1044) [NBP3-13699] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cadherin-11 antibody [HL1044] (NBP3-13699) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Cadherin-11 Antibody (HL1044) [NBP3-13699]-Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CDH11 antibody [HL1044] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Cadherin-11 Antibody (HL1044) [NBP3-13699] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Cadherin-11 antibody [HL1044] (NBP3-13699) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Cadherin-11 Antibody (HL1044) [NBP3-13699] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CDH11 antibody [HL1044] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu, Rt, Fe
• Applications: WB
• Clone: HL1044
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Cadherin-11 Antibody (HL1044) - Azide and BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Cadherin-11. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDH11
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cat (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Cadherin-11 Antibody (HL1044) - Azide and BSA Free

• CAD11OSF-4
• cadherin 11, type 2, OB-cadherin (osteoblast)
• Cadherin11
• Cadherin-11
• CDH11
• CDHOB
• OB
• OB-Cadherin
• Osteoblast cadherin

Background

Cadherin-11 is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. Cadherin-11 is expressed in osteoblasts and the brain as well as other tissues.Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH11