Human C-Reactive Protein/CRP ELISA Kit (Colorimetric)

ELISA: C-Reactive Protein/CRP ELISA Kit [HRP] [KA0238] - Standard curve

Product Discontinued

Human C-Reactive Protein/CRP ELISA Kit (Colorimetric) Summary

• Description: This ELISA kit has been transitioned to a new version, available here: DCRP00B
• Standard Curve Range: 0 to 0.10 mg/L
• Sensitivity: 0.1 mg/L
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 3.28%
• Intra-Assay: CV% < 4.44%
• Spike Recovery: 100.40%
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CRP

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human C-Reactive Protein/CRP ELISA Kit (Colorimetric)

• C-Reactive Protein
• C-reactive protein, pentraxin-related
• CRP
• MGC88244
• pentraxin 1
• PTX1MGC149895

Background

C Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis. Studies have revealed that among other markers of inflammation, CRP shows the strongest association with cardiovascular events. Many clinical studies demonstrated that coronary mortality among patients with unstable angina and elevated CRP is significantly higher comparing with the patients without elevated CRP. Measurements of C reactive protein (hsCRP) in the patients with ischemic heart disease provide a novel method for detecting individuals at high risk of plaque rupture.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for C-Reactive Protein/CRP ELISA Kit (KA0238)(14)

Publications using KA0238

• Maria A, E H, K A et al. Associations between metabolic factors and radiographic knee osteoarthritis in early disease - a cross-sectional study of individuals with knee pain. BMC Musculoskelet Disord. 2022-10-28 [PMID: 36307803]
• Maria A, Emma H, Katarina A et al. Cohort profile: the Halland osteoarthritis (HALLOA) cohort-from knee pain to osteoarthritis: a longitudinal observational study in Sweden. BMJ Open. 2022-07-14 [PMID: 35835523]
• Hussein MS, El-Barbary AM, Nada DW et al. Identification of serum interleukin-13 and interleukin-13 receptor subunit expressions: Rheumatoid arthritis-associated interstitial lung disease International journal of rheumatic diseases 2021-02-26 [PMID: 33638296] (ELISA)
• Gangadaran S, Cheema SK. A high fat diet enriched with sea cucumber gut powder provides cardio-protective and anti-obesity effects in C57BL/6 mice. Food Res Int 2017-06-28 [PMID: 28784546]
• Dekens DW, Naude PJ, Engelborghs S et al. Neutrophil Gelatinase-Associated Lipocalin and its Receptors in Alzheimer's Disease (AD) Brain Regions: Differential Findings in AD with and without Depression. J Alzheimers Dis 2016-11-19 [PMID: 27716662]
• Park S, Jung CW, Kim K et al. Iron deficient erythropoiesis might play key role in development of anemia in cancer patients. Oncotarget 2015-12-15 [PMID: 26517509] (ELISA)
• Ssinabulya I, Kayima J, Longenecker C et al. Subclinical atherosclerosis among HIV-infected adults attending HIV/AIDS care at two large ambulatory HIV clinics in Uganda. PLoS One. 2014-01-01 [PMID: 24586854] (ELISA)
• Ranadive SM, Kappus RM, Cook MD et al. Effect of acute moderate exercise on induced inflammation and arterial function in older adults. Exp Physiol. 2014-03-07 [PMID: 24465022]
• Bastrup-Birk S, Skjoedt MO, Munthe-Fog L et al. Pentraxin-3 serum levels are associated with disease severity and mortality in patients with systemic inflammatory response syndrome. PLoS One. 2013-01-01 [PMID: 24039869] (Human)
• Nantanda R, Ostergaard MS, Ndeezi G, Tumwine JK. Factors associated with asthma among under-fives in Mulago hospital, Kampala Uganda: a cross sectional study. BMC Pediatr. 2013-09-11 [PMID: 24024970]
• Liu Z, Zhou C, Liu Y et al. The expression levels of plasma micoRNAs in atrial fibrillation patients. PLoS One. 2012-01-01 [PMID: 23028671] (Human)
• Chen R, Mias GI, Li-Pook-Than J et al. Personal omics profiling reveals dynamic molecular and medical phenotypes. Cell. 2012-03-16 [PMID: 22424236]
• Esteban-Fernandez de Avila B, Escamilla-Gomez V, Campuzano S et al. Ultrasensitive amperometric magnetoimmunosensor for human C-reactive protein quantification in serum. Sensors and Actuators B: Chemical 2013-07-17 (ELISA, Human)
• Vaidya HB, Gangadaran S, Cheema SK. et al. A high fat-high sucrose diet enriched in blue mussels protects against systemic inflammation, metabolic dysregulation and weight gain in C57BL/6 mice. Food Res Int 2017-08-14 [PMID: 28888461]

FAQs for C-Reactive Protein/CRP ELISA Kit (KA0238). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CRP