C-Reactive Protein/CRP Antibody [Biotin]

C‑Reactive Protein/CRP was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human C‑Reactive Protein/CRP Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1707) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

C-Reactive Protein/CRP Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human C‑Reactive Protein/CRP
  Phe17-Pro224
  Accession # P02741
• Specificity: Detects C‑Reactive Protein/CRP in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse CRP is observed and 1% cross-reactivity with recombinant rat CRP is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CRP
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for C-Reactive Protein/CRP Antibody [Biotin]

• C-Reactive Protein
• C-reactive protein, pentraxin-related
• CRP
• MGC88244
• pentraxin 1
• PTX1MGC149895

Background

CRP is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are non-covalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively.

CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL-6, IL-1 and glucocorticoids are the major inducer of the CRP gene. In response to infection, inflammation or tissue damage, the level of CRP in human serum can increase 1,000-fold within 24‑48 hours. It will come back to base level of less than 1 μg/mL very fast. Human CRP is an acute-phase serum protein that plays a role in the first line in host innate host defense. Like other pentraxins, CRP exhibits Ca⁺⁺ - dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal walls, is a principal ligand of CRP. CRP also binds to the membrane of injured cells, membrane and nuclear components of necrotic and apoptotic cells. Upon binding with the ligands, CRP is recognized by C1q and initiates the activation of complement cascade. Ligand bound CRP also binds to Fc gamma RI and Fc gamma RIIa on phagocytes and activates phogocytotic responses. In addition to phogocytosis, CRP also can induce production of hydrogen peroxide and inflammatory cytokines, such as IL-1, IL-6 and TNF‑ alpha  by monocytes. With these functions, human CRP is an important serum protein for anti-bacterial pathogen and clearance of damaged and apoptotic cells. However, in mouse, CRP is expressed at very low level and is not an acute phase reactant. Serum amyloid P component (SAP), another pentraxin, is the major acute phase serum protein in mice. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.

1. Gotschlich, E.C. and G.M. Edelman (1965) Proc. Natl. Acad. Sci. USA 54:558.
2. Volanakis, J.E. (2001) Molecular Immunology 38:189.
3. Bharadwaj, D. et al. (1999) J. Experimental Medicine 190:585.
4. Ballou S.P. and G. Lozanski (1992) Cytokine 4:361.
5. Danesh, J. et al. (2004) N. Engl. J. Med. 350:1387.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C-Reactive Protein/CRP Antibody (BAF1707)(1)

Publication using BAF1707

• Newman, S;Wilson, B;Mamerow, D;Wollant, B;Nyein, H;Rosenberg-Hasson, Y;Maecker, H;Eisenstein, M;Soh, H; Extending the dynamic range of biomarker quantification through molecular equalization bioRxiv 2023-01-01 [PMID: 37443317] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CRP
• Uniprot:
  • P02741()