C-Reactive Protein/CRP Antibody (5A9) - BSA Free

Western Blot: C-Reactive Protein/CRP Antibody (5A9) [NBP1-04282] - C-Reactive Protein Antibody (5A9) Cell lysates of NIH-3T3, HepG2, Jurkat, HeLa and SH-SY5Y (each 40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: C-Reactive Protein/CRP Antibody (5A9) [NBP1-04282] - Analysis of CRP in HepG2 cells line.

Flow Cytometry: C-Reactive Protein/CRP Antibody (5A9) [NBP1-04282] - Analysis of CRP in HepG2 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Product Discontinued

C-Reactive Protein/CRP Antibody (5A9) - BSA Free Summary

• Immunogen: Recombinant human C-Reactive Protein/CRP (19-224aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRP
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for C-Reactive Protein/CRP Antibody (5A9) - BSA Free

• C-Reactive Protein
• C-reactive protein, pentraxin-related
• CRP
• MGC88244
• pentraxin 1
• PTX1MGC149895

Background

C-reactive protein (CRP) is an acute-phase protein with a well-known association with infection and other inflammatory conditions. Mild elevations in C-reactive protein concentration predict myocardial infarction, stroke, and vascular death in a variety of clinical settings. Despite the lack of specific evidence that C-reactive protein levels are independently associated with cardiovascular risk in patients with hypertension, the prognostic value of C-reactive protein has proven to be complementary to that of blood pressure values.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRP
• Entrez:
  • 1401(Human)
• Uniprot:
  • NP_000558(Human)