This assay has high sensitivity and excellent specificity for detection of cynomologus monkey C-Peptide. No significant cross-reactivity or interference between cynomologus monkey C-Peptide and analogues was observed.
Standard Curve Range
0.5 ng/mL - 8 ng/mL
Sensitivity
0.25 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 15
Intra-Assay
%CV < 15
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
INS
Applications/Dilutions
Dilutions
ELISA
Publications
Read Publications using NBP2-59957 in the following applications:
Storage of components varies. See protocol for specific instructions.
Kit Components
Components
Adhesive Strip (For 96 wells)
Assay plate
HRP-conjugate
Instruction manual
Standard
Stop Solution
Substrate A
Substrate B
Wash Buffer (20 x concentrate)
Alternate Names for Cynomolgus Monkey C-Peptide ELISA Kit (Colorimetric)
C-P
C-Peptide
IDDM2
ILPR
Insulin C-Peptide
insulin
IRDN
MODY10
Proinsulin C-Peptide
proinsulin
Background
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C-Peptide has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for C-Peptide. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C-Peptide bound in the initial step. The color development is stopped and the intensity of the color is measured.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.