Human C-Peptide ELISA Kit (Colorimetric) Summary
| Description |
C-Peptide ELISA Kit is a solid phase enzyme-linked immunosorbent assay for the quantitative in vitro diagnostic measurement of C-Peptide in serum, plasma and urine. |
| Standard Curve Range |
0.06 to 16 ng/mL |
| Assay Type |
solid phase enzyme-linked immunosorbent assay |
| Inter-Assay |
CV% < 9.21% |
| Intra-Assay |
CV% < 6.12% |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
INS |
Applications/Dilutions
| Dilutions |
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| Application Notes |
Useful for quantification. |
| Publications |
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Packaging, Storage & Formulations
Kit Components
|
Components
|
- Antiserum: Ready to use. Monoclonal mouse anti C-Peptide antibody contains non-mercurypreservative
- Enzyme Complex: Ready to use. Contains horseradish Peroxidase. Contains non-mercurypreservative.
- Enzyme Conjugate: Ready to use. Biotinylated C-Peptide contains non-mercury preservative
- Microtiterwells: 12 x 8 (break apart) strips; Wells coated with anti-mouse-antibody
- Sample Diluent: Ready to use, Contains non-mercury preservative
- Standard (Standard 0-5): Lyophilized, Concentrations 0-16 ng/mL (see exact value on theQC-Datasheet). The standards are calibrated against WHO approved Reference material IRRC-Peptide, code 84/510. Contain non-mercury preservative
- Stop Solution: Ready to use. Contains 1 N acidic solution. Avoid contact with the stopsolution. It may cause skin irritations and burns.
- Substrate Solution: Ready to use. TMB
- Wash Solution (40X concentrated)
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Human C-Peptide ELISA Kit (Colorimetric)
Background
C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Publications for C-Peptide ELISA Kit (KA1259)(3)
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Product General Protocols
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FAQs for C-Peptide ELISA Kit (KA1259). (Showing 1 - 2 of 2 FAQs).
-
Does your C-peptide ELISA kit (cat#KA1259) show cross-reactivity with monkey C-peptide?
- KA1259 has not been validated for use in monkeys. However, C-peptide of human shows greater than 96% similarity to macaque, orangutan, gorilla and chimpanzee. This kit is expected to cross-react.
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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