C-Peptide Antibody (SA0410)

Immunohistochemistry-Paraffin: C-Peptide Antibody (SA0410) [NBP2-67288] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Insulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: C-Peptide Antibody (SA0410) [NBP2-67288] - Analysis of paraffin-embedded human pancreas tissue using anti-Insulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: C-Peptide Antibody (SA0410) [NBP2-67288] - Analysis of paraffin-embedded mouse pancreas tissue using anti-Insulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: C-Peptide Antibody (SA0410) [NBP2-67288] - Analysis of paraffin-embedded rat pancreas tissue using anti-Insulin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: C-Peptide Antibody (SA0410) [NBP2-67288] - Immunofluorescence staining of paraffin- embedded mouse pancreas tissue using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/200 dilution for 10 hours at 4? and detected using Alexa Fluor (TM) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

Immunohistochemistry-Paraffin: C-Peptide Antibody (SA0410) [NBP2-67288] - Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Insulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: C-Peptide Antibody (SA0410) [NBP2-67288] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Insulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

C-Peptide Antibody (SA0410) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within human C-Peptide aa 15-110. (SwissProt: P01308 Human; SwissProt: P01325 Mouse; SwissProt: P01322 Rat)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: INS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:20000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for C-Peptide Antibody (SA0410)

• C-P
• C-Peptide
• IDDM2
• ILPR
• Insulin C-Peptide
• insulin
• IRDN
• MODY10
• Proinsulin C-Peptide
• proinsulin

Background

C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide and two long strands of amino acids (called the alpha and beta chains) that later become linked together to form the insulin molecule. From every molecule of proinsulin, one molecule of insulin plus one molecule of C Peptide are produced. C peptide is released into the blood stream in equal amounts to insulin. A test of C peptide levels will show how much insulin the body is making. Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INS