C-Peptide Antibody (2B7) - BSA Free

• Reactivity: Hu, Bv(-), Mu(-), Po(-), Rt(-)
• Applications: ELISA, Func
• Clone: 2B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

C-Peptide Antibody (2B7) - BSA Free Summary

• Description: Novus Biologicals Mouse C-Peptide Antibody (2B7) - BSA Free (NB110-2555) is a monoclonal antibody validated for use in ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Hybridoma clone has been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with recombinant C-peptide construction.
• Epitope: The three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.
• Localization: Secreted
• Specificity: Affinity (Kd) > 1 x 10^8. Binds to the human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by MAbs are in the three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.. No cross-reactivity with human, bovine, porcine and mouse/rat insulin.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: INS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000 Validated for Competitive ELISA
  • Radioimmunoassay 1:100-1:2000
  • Radioimmunodiffusion
• Application Notes: Competitive ELISA or RIA detection of C-peptide in human serum without or after immunospecific removal of proinsulin (with solid-phased ab8299 (1D6) to human proinsulin)Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assays for the measurement of total (including proinsulin convertion intermediates) human proinsulin in serum. ab8297 used in conjunction with this antibody would be suitable.Specific immunosorbtional (with solid-phase 2B7) removal of total proinsulin and C-peptide molecules from serum samples before the measurement of human insulin

Reactivity Notes

Cross-reacts with Human. Does not react with Bovine, Mouse, Rat or Pig

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for C-Peptide Antibody (2B7) - BSA Free

• C-P
• C-Peptide
• IDDM2
• ILPR
• Insulin C-Peptide
• insulin
• IRDN
• MODY10
• Proinsulin C-Peptide
• proinsulin

Background

This antibody detects human Insulin and a different epitope than the monoclonal, ab8297.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INS