C-Peptide Antibody (1H8)

Product Discontinued

C-Peptide Antibody (1H8) Summary

• Immunogen: This C-Peptide antibody is made to a recombinant full length protein.
• Epitope: The three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.
• Localization: Secreted
• Specificity: Binds to the human free C-peptide and proinsulin molecules. No cross reactivity with human, bovine, porcine insulins. Affinity (Kd) > 1 x 108
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: INS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Radioimmunoassay 1:100 - 1:2000
• Application Notes: Competitive ELISA or RIA detection of C-peptide in human serum without or after immunospecific removal of proinsulin (with solid-phased ab8299 (1D6) to human proinsulin)Construction of a sensitive two-site sandwich EIA/ RIA immunosorbent assays for the measurement of total (including proinsulin convertion intermediates) human proinsulin in serum. ab8298 (2B7) used in conjunction with this antibody would be suitable. Specific immunosorbtional (with solid-phase 1H8) removal of total proinsulin and C-peptide molecules from serum samples before the measurement of human insulin.

Reactivity Notes

No cross-reactivity with human, bovine, porcine and mouse/rat insulin. All MAbs bind with equal efficiency to the human free C-peptide and C-peptide region in proinsulin molecules. Antigenic determinants identified by MAbs are in the three-dimensional structure generated by N- and C- terminal regions of C-peptide separated by beta-turn at position 47-50 of proinsulin.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for C-Peptide Antibody (1H8)

• C-P
• C-Peptide
• IDDM2
• ILPR
• Insulin C-Peptide
• insulin
• IRDN
• MODY10
• Proinsulin C-Peptide
• proinsulin

Background

This antibody detects a different epitope than the monoclonal, ab8298.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: INS