c-Myc Antibody (9E10) [Phycoerythrin]

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc PE-conjugated Monoclonal Antibody (Catalog # IC3696P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 9E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

c-Myc Antibody (9E10) [Phycoerythrin] Summary

• Immunogen: C-terminal region peptide of human c-Myc
  Ala408-Ala439
  Accession # P01106
• Specificity: Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYC
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for c-Myc Antibody (9E10) [Phycoerythrin]

• avian myelocytomatosis viral oncogene homolog
• BHLHE39
• bHLHe39MRTL
• Class E basic helix-loop-helix protein 39
• cMyc
• c-Myc
• myc proto-oncogene protein
• Myc
• Myc2
• MYCC
• myc-related translation/localization regulatory factor
• Niard
• Nird
• Proto-oncogene c-Myc
• Transcription factor p64
• v-myc avian myelocytomatosis viral oncogene homolog
• v-myc myelocytomatosis viral oncogene homolog (avian)

Background

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max.  It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for c-Myc Antibody (IC3696P)(8)

Publications using IC3696P

• Park, JH;Kim, SJ;Kim, OH;Kim, DJ; Enhanced Efficacy of Gastric Cancer Treatment through Targeted Exosome Delivery of 17-DMAG Anticancer Agent International journal of molecular sciences 2024-08-12 [PMID: 39201449] (Flow Cytometry, Human)
• Jiao, D;Sun, R;Ren, X;Wang, Y;Tian, P;Wang, Y;Yuan, D;Yue, X;Wu, Z;Li, C;Gao, L;Ma, C;Liang, X; Lipid accumulation-mediated histone hypoacetylation drives persistent NK cell dysfunction in anti-tumor immunity Cell reports 2023-10-03 [PMID: 37792534] (Flow Cytometry, Human)
• Jack E. Bowyer, Deboki Chakravarti, Wilson W. Wong, Declan G. Bates Mechanistic modelling of tyrosine recombination reveals key parameters determining the performance of a CAR T cell switching circuit Engineering Biology 3/24/2020 [PMID: 36970230]
• S Kakuda, RK Lopilato, A Ito, RS Haltiwange Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2 J. Biol. Chem., 2020-08-19;0(0):. 2020-08-19 [PMID: 32820046] (Flow Cytometry, Human)
• SR Morris, B Chen, JC Mudd, S Panigrahi, CL Shive, SF Sieg, CM Cameron, DA Zidar, NT Funderburg, SA Younes, B Rodriguez, S Gianella, MM Lederman, ML Freeman "Inflammescent" CX3CR1+CD57+ CD8 T cells are generated and expanded by IL-15 JCI Insight, 2020-06-04;0(0):. 2020-06-04 [PMID: 32369455] (Human)
• A Pandey, BM Harvey, MF Lopez, A Ito, RS Haltiwange, H Jafar-Neja Glycosylation of Specific Notch EGF Repeats by O-Fut1 and Fringe Regulates Notch Signaling in Drosophila Cell Rep, 2019-11-12;29(7):2054-2066.e6. 2019-11-12 [PMID: 31722217] (Flow Cytometry, Drosophila melanogaster)
• Deboki Chakravarti, Leidy D. Caraballo, Benjamin H. Weinberg, Wilson W. Wong Inducible Gene Switches with Memory in Human T Cells for Cellular Immunotherapy ACS Synthetic Biology 8/16/2019 [PMID: 31268301]
• GK Zakiryanov, E Kustova, NT Urazalieva, ET Baimuchame, NN Nakisbekov, MR Shurin Abnormal Expression of c-Myc Oncogene in NK Cells in Patients with Cancer Int J Mol Sci, 2019-02-11;20(3):. 2019-02-11 [PMID: 30754645] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: MYC
• Uniprot:
  • P01106()