BubR1 Antibody (OTI6E5) - Azide and BSA Free

Western Blot: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BubR1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BubR1.

Immunocytochemistry/Immunofluorescence: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY BubR1.

Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded pancreas tissue using anti-BubR1 mouse monoclonal antibody.

Flow Cytometry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - HEK293T cells transfected with either pCMV6-ENTRY BubR1.(Red) or empty vector control plasmid (Blue) were immunostaining with anti-BubR1 mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BubR1 mouse monoclonal antibody.

Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BubR1 mouse monoclonal antibody.

Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-BubR1 mouse monoclonal antibody.

Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded colon tissue using anti-BubR1 mouse monoclonal antibody.

Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded endometrium tissue using anti-BubR1 mouse monoclonal antibody.

Immunohistochemistry: BubR1 Antibody (OTI6E5) - Azide and BSA Free [NBP2-70314] - Staining of paraffin-embedded prostate tissue using anti-BubR1 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI6E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

BubR1 Antibody (OTI6E5) - Azide and BSA Free Summary

• Immunogen: Full-length protein expressed in 293T cell transfected with human BUB1B expression vector
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BUB1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200 - 1:1000
• Theoretical MW: 119.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for BubR1 Antibody (OTI6E5) - Azide and BSA Free

• Bub1A
• BUBR1budding uninhibited by benzimidazoles 1 (yeast homolog), beta
• budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• EC 2.7.11.1
• hBUBR1
• MAD3/BUB1-related protein kinase
• MAD3LBUB1beta
• Mitotic checkpoint kinase MAD3L
• Protein SSK1
• SSK1mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Background

Mitotic checkpoint serine/threonine-protein kinase BUB1 beta (BUB1B), also known as BubR1, is a kinase involved in spindle checkpoint function. BUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. BubR-1 localizes to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation.

BubR1 antibodies are useful tools for cancer research and cell division studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BUB1B