Bub1 Antibody - BSA Free

Western Blot: Bub1 Antibody [NBP1-76797] - A-20 lysate with bub1 antibody at (A) 2 and (B) 4 ug/ml.

Immunocytochemistry/Immunofluorescence: Bub1 Antibody [NBP1-76797] - A20 cells with Bub1 antibody at 10 ug/ml.

Immunocytochemistry/ Immunofluorescence: Bub1 Antibody - BSA Free [NBP1-76797] - Immunofluorescence of Bub1 in A20 cells with Bub1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Bub1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Bub1 Antibody - BSA Free (NBP1-76797) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1. The immunogen is located within amino acids 40 - 90 of Bub1. Amino Acid Squence: KEFLDKKKYHNDPRFISY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BUB1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10-20 ug/ml
  • Western Blot 2-4 ug/ml
• Control Peptide:

  Bub1 Peptide (NBP1-76797PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Bub1 Antibody - BSA Free

• BUB1 budding uninhibited by benzimidazoles 1 homolog
• Bub1
• BUB1Amitotic checkpoint serine/threonine-protein kinase BUB1
• BUB1L
• budding uninhibited by benzimidazoles 1 (yeast homolog)
• budding uninhibited by benzimidazoles 1 homolog (yeast)
• EC 2.7.11.1
• hBUB1mitotic spindle checkpoint kinase
• putative serine/threonine-protein kinase

Background

Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BUB1
• Uniprot:
  • O43683()