THRAP3 Antibody

Images

 
Independent Antibodies: Western Blot: THRAP3 Antibody [NB100-40848] - Detection of Human THRAP3/TRAP150 on HeLa whole cell lysate using NB100-40848. THRAP3/TRAP150 was also immunoprecipitated by other rabbit ...read more
Immunohistochemistry-Paraffin: THRAP3 Antibody [NB100-40848] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- THRAP3/TRAP150 used at a dilution of 1:1,000 (0.2ug/ml). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

THRAP3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 905 and the C-terminus (residue 955) of human Thyroid Hormone Receptor Associated Protein 3 using the numbering given in entry NP_005110.1 (GeneID 9967).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
THRAP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500- 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
Application Notes
Use in chromatin immunoprecipitation reported in scientific literature (PMID 24819052). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Knockdown validation (PMID: 31890047).
Publications
Read Publications using
NB100-40848 in the following applications:

  • 1 publication
  • KD
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for THRAP3 Antibody

  • FLJ22082
  • MGC133082
  • MGC133083
  • thyroid hormone receptor associated protein 3
  • Thyroid hormone receptor-associated protein complex 150 kDa component
  • thyroid hormone receptor-associated protein, 150 kDa subunit
  • Trap150
  • TRAP150thyroid hormone receptor-associated protein 3

Background

Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TR alpha and TR beta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3 triiodo L thyronine (T3). Thyroid hormones, through their interaction with the thyroid receptor (TR), effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for THRAP3 Antibody (NB100-40848)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ChIP, KD, WB.


Filter By Application
ChIP
(1)
KD
(1)
WB
(2)
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Human
(2)
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Product General Protocols

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Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for THRAP3 Antibody (NB100-40848) (0)

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Bioinformatics

Gene Symbol THRAP3
Entrez
Uniprot