THRAP3 Antibody

Western Blot: THRAP3 Antibody [NB100-40848] - Detection of Human THRAP3/TRAP150 on HeLa whole cell lysate using NB100-40848. THRAP3/TRAP150 was also immunoprecipitated by other rabbit anti-THRAP3/TRAP150 antibodies and NB100-40847.

Immunohistochemistry-Paraffin: THRAP3 Antibody [NB100-40848] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- THRAP3/TRAP150 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Western Blot: THRAP3 Antibody [NB100-40848] - ORF2p chA1-L1 antibody cross-reacts with BCLAF1. LNCaP cells were treated with Scramble, BCLAF1 (a) or THRAP3 (b) siRNA for 72 h. Whole cell lysates were assayed via western blot & probed with chA1-L1 ORF2p antibody, BCLAF1 or THRAP3, ORF1p, & Tubulin (loading control). Intensity of ORF1 bands in b were normalized to tubulin levels. c) Left: BCLAF1 Tagged overexpression construct with N-terminus 3x flag, V5, & Venus tag (~ 35 kDa). Right: HEK293 cells were transfected with vector only (VO) or tagged BCLAF1 construct. Whole cell lysates were assayed by western blot & probed with ORF2p antibody chA1-L1, & Tubulin (loading control) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31890047), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu
• Applications: WB, IHC, IP, ChIP, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

THRAP3 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 905 and the C-terminus (residue 955) of human Thyroid Hormone Receptor Associated Protein 3 using the numbering given in entry NP_005110.1 (GeneID 9967).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THRAP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000-1:10000
• Application Notes: Use in chromatin immunoprecipitation reported in scientific literature (PMID 24819052). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Knockdown validation (PMID: 31890047).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for THRAP3 Antibody

• FLJ22082
• MGC133082
• MGC133083
• thyroid hormone receptor associated protein 3
• Thyroid hormone receptor-associated protein complex 150 kDa component
• thyroid hormone receptor-associated protein, 150 kDa subunit
• Trap150
• TRAP150thyroid hormone receptor-associated protein 3

Background

Thyroid hormone receptors (TRs) are ligand dependent members of the steroid/retinoic acid superfamily of transcription factors. The two genes encoding TRs identified to date, TR alpha and TR beta, have been mapped to human chromosomes 17 and 3, respectively. TRs bind to thyroid hormone response elements (TREs) with half site binding motifs in the orientation of palindromes, direct repeats, or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3 triiodo L thyronine (T3). Thyroid hormones, through their interaction with the thyroid receptor (TR), effect metabolic processes, growth and development in many tissues by regulating the expression of genes for growth hormone, malic enzyme and several hepatic proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for THRAP3 Antibody (NB100-40848)(4)

Publications using NB100-40848

• Rawat C, Ben-Salem S, Singh N et al. Prostate cancer progression relies on the mitotic kinase citron kinase Cancer research 2023-10-06 [PMID: 37801613]
• Sigismondo G, Arseni L, Palacio-Escat N et al. Multi-layered chromatin proteomics identifies cell vulnerabilities in DNA repair Nucleic acids research 2023-01-25 [PMID: 36629267] (PLA, ICC/IF, Human)
• Briggs EM, Spadafora C, Logan SK A re-evaluation of LINE-1 ORF2 expression in LNCaP prostate cancer cells Mob DNA 2019-12-29 [PMID: 31890047] (KD, WB, Human)
• Diefenbacher ME, Reich D, Dahley O et al. The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters. PLoS ONE. 2014-05-13 [PMID: 24819052] (WB, Chemotaxis, Human)

Bioinformatics

• Gene Symbol: THRAP3
• Entrez:
  • 9967(Human)
• Uniprot:
  • Q9Y2W1(Human)