Emerin Antibody

Western Blot: Emerin Antibody [NBP1-87692] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: Emerin Antibody [NBP1-87692] - Staining of human cell line U-251 MG shows localization to nuclear membrane & endoplasmic reticulum. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Emerin Antibody [NBP1-87692] - Staining of human skeletal muscle shows moderate nuclear membrane positivity in myocytes.

Western Blot: Emerin Antibody [NBP1-87692] - Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

• Reactivity: Hu, Mu, Rt, Ha
• Applications: WB, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Emerin Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ASSYSFSDLNSTRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSACQSITHY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EMD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB reported in scientific literature (PMID: 27854297). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF IFixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  Emerin Protein (NBP1-87692PEP)

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 27548707).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Emerin Antibody

• emerin
• Emery-Dreifuss muscular dystrophy
• LEM domain containing 5
• STAEDMDLEMD5

Background

Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Emerin Antibody (NBP1-87692)(5)

Publications using NBP1-87692

• Porter LJ, Holt MR, Soong D et al. Prelamin A Accumulation Attenuates Rac1 Activity and Increases the Intrinsic Migrational Persistence of Aged Vascular Smooth Muscle Cells. Cells. 2016-11-15 [PMID: 27854297] (WB, Human)
• Tajik A, Zhang Y, Wei F et al. Transcription upregulation via force-induced direct stretching of chromatin. Nat Mater. 2016-08-22 [PMID: 27548707] (WB, Hamster)
• Collard L, Herledan G, Pincini A et al. Nuclear actin and Mrtfs control disuse muscle atrophy via Srf activity regulation. J. Cell. Sci. 2014-10-24 [PMID: 25344251]
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]
• Lomnytska MI, Becker S, Gemoll T et al. Impact of genomic stability on protein expression in endometrioid endometrial cancer. Br J Cancer 2012-03-27 [PMID: 22415234]

Bioinformatics

• Gene Symbol: EMD
• Entrez:
  • 2010(Human)
• Uniprot:
  • P50402(Human)