BTF Antibody

Western Blot: BTF Antibody [NB100-1704] - Whole cell lysate from HeLa (5, 15, 50 ug loaded) and 293T cells (T; 50 ug) probed with anti-BTF antibody at 0.04 ug/ml.

Immunohistochemistry: BTF Antibody [NB100-1704] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-BTF used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.

Western Blot: BTF Antibody [NB100-1704] - : Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells.

Immunoprecipitation: BTF Antibody [NB100-1704] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

BTF Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 150 and 200 of human BCL2-Associated Transcription Factor 1 using the numbering given in entry NP_055554.1 (GeneID 9774).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCLAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1 to 4 ug/mg of lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BTF Antibody

• bcl-2-associated transcription factor 1
• BCL2-associated transcription factor 1
• Btf
• BTFKIAA0164bK211L9.1

Background

BTF is a transcriptional repressor that interacts with Bcl-2 related proteins to promote apoptosis. BTF has also been found to interact with emerin, a nuclear protein implicated in the muscle wasting disease Emery-Dreifuss muscular dystrophy. Alternate designations for BTF include Bcl-2-associated transcription factor 1, BCLAF1, KIAA0164, and bK211L9.1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTF Antibody (NB100-1704)(4)

Publications using NB100-1704

• Lee YY, Yu YB, Gunawardena HP et al. BCLAF1 is a radiation-induced H2AX-interacting partner involved in GH2AX-mediated regulation of apoptosis and DNA repair. Cell Death Dis. 2012-07-26 [PMID: 22833098]
• Lee SH, Kalejta RF, Kerry J et al. BclAF1 restriction factor is neutralized by proteasomal degradation and microRNA repression during human cytomegalovirus infection. Proc Natl Acad Sci U S A 2012-06-01 [PMID: 22645331]
• Ziegelbauer JM, Sullivan CS, Ganem D et al. Tandem array-based expression screens identify host mRNA targets of virus-encoded microRNAs. Nat Genet 2009-01-01 [PMID: 19098914]
• Liu H, Lu ZG, Miki Y et al. Protein kinase C delta induces transcription of the TP53 tumor suppressor gene by controlling death-promoting factor Btf in the apoptotic response to DNA damage. Mol Cell Biol 2007-12-01 [PMID: 17938203]

Bioinformatics

• Gene Symbol: BCLAF1
• Entrez:
  • 9774(Human)
• Uniprot:
  • NP_055554.1(Human)