BRSK1 Antibody - BSA Free

Western Blot: BRSK1 Antibody [NBP1-77280] - Human brain tissue lysate with BRSK1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

Immunocytochemistry/ Immunofluorescence: BRSK1 Antibody - BSA Free [NBP1-77280] - Immunofluorescence of BRSK1 in Human Brain cells with BRSK1 antibody at 20 u/mL.

Immunohistochemistry: BRSK1 Antibody - BSA Free [NBP1-77280] - Immunohistochemistry of BRSK1 in human brain tissue with BRSK1 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BRSK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BRSK1 Antibody - BSA Free (NBP1-77280) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 28 amino acid synthetic peptide from near the center of human BRSK1. The immunogen is located within amino acids 450 - 500 of BRSK1. Amino Acid Squence: DEARGGGSPTSKTQTLPSRGPRGGGAGE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BRSK1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Control Peptide:

  BRSK1 Peptide (NBP1-77280PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for BRSK1 Antibody - BSA Free

• BR serine/threonine kinase 1
• EC 2.7.11
• EC 2.7.11.1
• FLJ43009
• KIAA1811BR serine/threonine-protein kinase 1
• protein kinase SAD1A
• SAD1 kinase
• SAD1
• Serine/threonine-protein kinase SAD-B

Background

BRSK1 was initially identified as a mammalian homolog to the fission yeast S. pombe Cdr2, a mitosis-regulatory kinase and also shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK1 is unbiquitously expressed, with highest levels of expression in the brain and testes. Similar to its yeast homolog, BRSK1 is thought to be involved in stress-induced cell cycle arrest. Overexpression of this protein leads to the G2/M arrest in HeLa S2 cells and UV-induced G2/M arrest could be partially abrogated by reduced expression of BRSK1 through the use of siRNA, indicating its role in DNA damage checkpoint function. More recently, it has been shown that both BRSK1 and the related protein BRSK2 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. At least two isoforms of BRSK1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BRSK1
• Uniprot:
  • Q8TDC3(Human)