BRSK2 Antibody

Images

 
Western Blot: BRSK2 Antibody [NBP1-77281] - Analysis of BRSK2 in human brain tissue lysate with BRSK2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.
Immunohistochemistry: BRSK2 Antibody [NBP1-77281] - BRSK2 in human brain tissue with BRSK2 antibody at 5 ug/mL.
Immunofluorescence: BRSK2 Antibody [NBP1-77281] - BRSK2 in Human Brain cells with BRSK2 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BRSK2 Antibody Summary

Immunogen
Antibody was raised against a 23 amino acid synthetic peptide from near the carboxy terminus of human BRSK2. The immunogen is located within amino acids 630 - 680 of BRSK2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRSK2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunofluorescence
Control Peptide
BRSK2 Peptide (NBP1-77281PEP)

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Rat: (87%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRSK2 Antibody

  • BR serine/threonine kinase 2
  • BR serine/threonine-protein kinase 2
  • C11orf7
  • chromsosome 11 open reading frame 7
  • EC 2.7.11
  • EC 2.7.11.1
  • FLJ41362
  • PEN11BSAD1
  • protein kinase SAD1B
  • serine/threonine kinase 29
  • serine/threonine kinase SAD-A
  • Serine/threonine-protein kinase 29
  • Serine/threonine-protein kinase SAD-A
  • STK29

Background

BRSK2 was initially identified through a computer screen of the human genome and shows significant homology to the C. elegans neuronal cell polarity regulator SAD1. BRSK2 is expressed in the brain and to a lesser extent in the testes. BRSK2 is a member of the AMP-activated protein kinase subfamily and can be activated by the tumor suppressor kinase LKB1. More recently, it has been shown that both BRSK2 and the related protein BRSK1 are required for mammalian neuronal polarization. While BRSK1- and BRSK2-null mice were viable, double-mutant mice died within two hours of birth. Neurons from these mice showed uniformly-sized neurites as opposed to the normal long axon and multiple shorter dendrites. These neurites also displayed both axonal and dendritic markers. BRSK2 has also been shown to be an autoantigen in paraneoplastic limbic encephalitis. At least four isoforms of BRSK2 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BRSK2
Uniprot