BRD3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: BRD3 Antibody [NBP2-15612] - BRD3 antibody detects BRD3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD3 stained by BRD3 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.

Western Blot: BRD3 Antibody [NBP2-15612] - Sample (20 ug of whole cell lysate) A: mouse Liver 7. 5% SDS PAGE gel, diluted at 1:20000.

Immunohistochemistry-Paraffin: BRD3 Antibody [NBP2-15612] - BRD3 antibody detects BRD3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.BRD3 stained by BRD3 antibody (NBP2-15612) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: BRD3 Antibody [NBP2-15612] - HeLa whole cell and nuclear extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRD3 antibody (NBP2-15612) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BRD3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BRD3 Antibody - BSA Free (NBP2-15612) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human BRD3. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (93%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BRD3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000
• Theoretical MW: 80 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for BRD3 Antibody - BSA Free

• BRD3
• bromodomain containing 3
• bromodomain-containing protein 3
• KIAA0043FLJ41328
• ORFX
• ORFXbromodomain-containing 3
• RING3L
• RING3LFLJ23227
• RING3-like protein

Background

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BRD3
• Uniprot:
  • Q15059(Human)