BRD4 Antibody (BL-149-2H5)

Images

 
Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 (SK) lysate. Antibody: Rabbit anti-BRD4 recombinant ...read more
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393). Secondary: DyLight 550-conjugated goat anti-rabbit IgG. ...read more
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Chromatin Immunoprecipitation Sequencing: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq. Antibody: Rabbit anti-BRD4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, CHIP-SEQ, ICC/IF
Clone
BL-149-2H5
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BRD4 Antibody (BL-149-2H5) Summary

Immunogen
Immunogen sequence between amino acids 1312 and 1362
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BRD4
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:25-1:250
  • Immunohistochemistry 1:25 - 1:250
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 5 - 20 ul/0.5 mg lysate
  • Chromatin Immunoprecipitation Sequencing
  • Immunocytochemistry 1:25 - 1:250

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Antigen Affinity-purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for BRD4 Antibody (BL-149-2H5)

  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1bromodomain-containing protein 4
  • HUNKI
  • MCAP
  • Protein HUNK1

Background

Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional BRD4 Products

Bioinformatics Tool for BRD4 Antibody (NBP2-76393)

Discover related pathways, diseases and genes to BRD4 Antibody (NBP2-76393). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for BRD4 Antibody (NBP2-76393)

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Blogs on BRD4.

Epigenetic Control of Autophagy
By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studied....  Read full blog post.

Understanding the relationship between NUT and BET proteins in NMC
NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.

NUT - A Protein Coding Gene
The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating...  Read full blog post.

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Bioinformatics

Gene Symbol BRD4