BRD4 Antibody (BL-149-2H5)


Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of mouse BRD4 by WB of BW5147.3 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393). Secondary: DyLight 550-conjugated goat anti-rabbit IgG. more
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Section of breast carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-149-2H5] used at 1:250. Secondary: HRP-conjugated goat more
Flow Cytometry: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: BRD4 Antibody (BL-149-2H5) or isotype control (unshaded). Secondary: DyLight(R) more
Chromatin Immunoprecipitation Sequencing: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Localization of human BRD4 binding sites in immunoprecipitates from Mia PaCa-2 cells by ChIP-Seq. Antibody: Rabbit anti-BRD4 more
Western Blot: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of HeLa, HEK293T, Jurkat, MDA-MB-231, A-549, SW620, and SK-MEL-28 lysate. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: more
Immunohistochemistry-Paraffin: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 in FFPE Hela cells by ICC. Antibody: BRD4 Antibody (BL-149-2H5). Secondary: HRP-conjugated goat anti-rabbit IgG. more
Immunoprecipitation: BRD4 Antibody (BL-149-2H5) [NBP2-76393] - Detection of human BRD4 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-BRD4 recombinant monoclonal [BL-149-2H5] (NBP2-76393).

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CHIP-SEQ

Order Details

BRD4 Antibody (BL-149-2H5) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen sequence between amino acids 1312 and 1362
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation Sequencing
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000
Application Notes
Flow Cytometry Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ChIP-Seq 10 - 40 ul per 30 ug chromatin.

Packaging, Storage & Formulations

Store at 2 - 8 C / 1 year from date of receipt
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
0.09% Sodium Azide
Antigen Affinity-purified


This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for BRD4 Antibody (BL-149-2H5)

  • BRD4
  • bromodomain containing 4
  • bromodomain-containing 4
  • CAPchromosome-associated protein
  • HUNK1
  • HUNK1bromodomain-containing protein 4
  • MCAP
  • Protein HUNK1


Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Epigenetic Control of Autophagy
By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studi...  Read full blog post.

Understanding the relationship between NUT and BET proteins in NMC
NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.

NUT - A Protein Coding Gene
The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating...  Read full blog post.

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Gene Symbol BRD4