BRD3 Antibody (BLR069G)

Images

 
Western Blot: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.
Immunocytochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Immunohistochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Detection of human BRD3 FFPE colon carcinoma by IHC. Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] NBP2-77359. Secondary: DyLight® ...read more
Immunohistochemistry: BRD3 Antibody (BLR069G) [NBP2-77359] - Antibody: Rabbit anti-BRD3 recombinant monoclonal [BLR069G] (NBP2-77359). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
BLR069G
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BRD3 Antibody (BLR069G) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Immunogen was a peptide representing a region between residue 676 and 726 of human Bromodomain-containing protein 3 using the numbering given in entry NP_031397.1 (Gene ID 8019).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BRD3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 20 ul/mg lysate
  • Western Blot 1:1000
Application Notes
IHC-P: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

ICC/IF: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Packaging, Storage & Formulations

Storage
Store at 2 - 8 C / 1 year from date of receipt
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA
Preservative
0.09% Sodium Azide
Purity
Affinity purified

Notes

This Product sold under License from Bethyl Laboratories, Inc.

Alternate Names for BRD3 Antibody (BLR069G)

  • BRD3
  • bromodomain containing 3
  • bromodomain-containing protein 3
  • KIAA0043FLJ41328
  • ORFX
  • ORFXbromodomain-containing 3
  • RING3L
  • RING3LFLJ23227
  • RING3-like protein

Background

BRD3 was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BRD3 Antibody (NBP2-77359) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BRD3 Antibody (NBP2-77359)

Discover related pathways, diseases and genes to BRD3 Antibody (NBP2-77359). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for BRD3 Antibody (NBP2-77359)

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Blogs on BRD3.

Understanding the relationship between NUT and BET proteins in NMC
NUT has been found to fuse with bromodomain-containing proteins 3 and 4 (BRD3 and BRD4) in NUT midline carcinoma (NMC), a very rare, extremely aggressive, and genetically defined human cancer. NMC has recently been designated as a sub classificati...  Read full blog post.

NUT - A Protein Coding Gene
The NUT gene is found on chromosome 15q14 and encodes for the NUT protein which is a key component of the RNA polymerase II Mediator complex. This multi-subunit assembly is required for all RNA pol II-dependent transcriptional activation, coordinating...  Read full blog post.

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Bioinformatics

Gene Symbol BRD3