BRCC3 Antibody

Images

 
Western Blot: BRCC3 Antibody [NBP1-76831] - Analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: BRCC3 Antibody [NBP1-76831] - Immunofluorescence of BRCC36 in Human Brain cells with BRCC36 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: BRCC3 Antibody [NBP1-76831] - Human brain tissue with BRCC3 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

BRCC3 Antibody Summary

Immunogen
Antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. The immunogen is located within amino acids 20 - 70 of BRCC36.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRCC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 2 ug/mL
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin 2.5 ug/mL
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
BRCC3 Peptide (NBP1-76831PEP)

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Bovine: (76%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRCC3 Antibody

  • BRCA1/BRCA2-containing complex subunit 3
  • BRCA1/BRCA2-containing complex subunit 36
  • BRCA1/BRCA2-containing complex, subunit 3
  • BRCA1-A complex subunit BRCC36
  • BRCC36C6.1ACXorf53
  • BRISC complex subunit BRCC36
  • c6.1A
  • chromosome X open reading frame 53
  • EC 3.1.2.15
  • EC 3.4.19.-
  • lys-63-specific deubiquitinase BRCC36

Background

Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol BRCC3
Uniprot