BRCC3 Antibody - BSA Free

Western Blot: BRCC3 Antibody [NBP1-76831] - Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.ppat.1007071) licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [NBP1-76831] - Representative IF images showing BRCC3 signal in LT versus ST and MPPs. Scale bar 5 um. m Quantitation of BRCC3 IF signal from l in Msi2 WT and Msi2 K/O. n = 258; 263; 216 and 295 cells for LT Msi2 WT; KO; ST, MPPs Msi2 WT and KO, respectively. Data as mean +/- SEM. Unpaired Student t test, ****p < 0.0001. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15814-8) licensed under a CC-BY license.

Western Blot: BRCC3 Antibody [NBP1-76831] - Analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 ug/mL.

Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat delta K (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat delta K (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: BRCC3 Antibody - BSA Free [NBP1-76831] - Immunohistochemistry of BRCC3 in human brain tissue with BRCC3 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: BRCC3 Antibody - BSA Free [NBP1-76831] - Immunofluorescence of BRCC3 in Human Brain cells with BRCC3 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BRCC3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BRCC3 Antibody - BSA Free (NBP1-76831) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-BRCC3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human BRCC36. The immunogen is located within amino acids 20 - 70 of BRCC36. Amino Acid Squence: CIGELNDDTRSDSKFAY
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BRCC3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Knockdown Validated
  • Western Blot 0.5-2 ug/ml
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  BRCC3 Peptide (NBP1-76831PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for BRCC3 Antibody - BSA Free

• BRCA1/BRCA2-containing complex subunit 3
• BRCA1/BRCA2-containing complex subunit 36
• BRCA1/BRCA2-containing complex, subunit 3
• BRCA1-A complex subunit BRCC36
• BRCC36C6.1ACXorf53
• BRISC complex subunit BRCC36
• c6.1A
• chromosome X open reading frame 53
• EC 3.1.2.15
• EC 3.4.19.-
• lys-63-specific deubiquitinase BRCC36

Background

Together with the breast and ovarian predisposition proteins BRCA1 and BRCA2 and RAD51 and BRCC45, BRCC36 forms a holoenzyme complex that possesses a ubiquitin E3 ligase activity. Aberrant levels of BRCC36 were detected in sporadic breast tumors and depletion of either BRCC36 or BRCC45 by siRNA resulted in increased sensitivity to ionizing radiation and defects in the G2/M checkpoint, indicating that BRCC36 acts to enhance cellular survival following DNA damage. Recent experiments have shown that BRCC36 is essential for ionizing radiation-induced BRCA1 phosphorylation and nuclear foci formation, suggesting that BRCC36 may be an important target in the treatment of radiation-resistant breast tumors. At least two isoforms of BRCC36 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BRCC3 Antibody (NBP1-76831)(2)

Publications using NBP1-76831

• Nguyen DTT, Lu Y, Chu KL et al. HyperTRIBE uncovers increased MUSASHI-2 RNA binding activity and differential regulation in leukemic stem cells Nat Commun 2020-04-24 [PMID: 32332729] (ICC/IF, Mouse)
• Xu M, Moresco JJ, Chang M et al. SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy PLoS Pathog. 2018-05-23 [PMID: 29791506] (WB, Human)

Bioinformatics

• Gene Symbol: BRCC3
• Uniprot:
  • NP_077308(Human)