BRCC3 Antibodies
We offer BRCC3 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each BRCC3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our BRCC3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the BRCC3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our BRCC3 polyclonal antibodies and browse our BRCC3 monoclonal antibody catalog.
Alternate Names for BRCC3 Antibodies
anti-BRCC3 antibody, anti-BRCA1-A complex subunit BRCC36 antibody, anti-BRCA1/BRCA2-containing complex subunit 3 antibody, anti-BRCA1/BRCA2-containing complex subunit 36 antibody, anti-BRCA1/BRCA2-containing complex, subunit 3 antibody, anti-BRCC36C6.1ACXorf53 antibody, anti-BRISC complex subunit BRCC36 antibody, anti-c6.1A antibody, anti-chromosome X open reading frame 53 antibody, anti-EC 3.1.2.15 antibody, anti-EC 3.4.19.- antibody, anti-lys-63-specific deubiquitinase BRCC36 antibody
6 Results for "brcc3" in Primary Antibodies
![Western Blot: BRCC3 Antibody [NBP1-76831] - Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.ppat.1007071) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Knockdown-Validated-NBP1-76831-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BRCC3 Antibody [NBP1-76831] - Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.ppat.1007071) licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [NBP1-76831] - Representative IF images showing BRCC3 signal in LT versus ST and MPPs. Scale bar 5 um. m Quantitation of BRCC3 IF signal from l in Msi2 WT and Msi2 K/O. n = 258; 263; 216 and 295 cells for LT Msi2 WT; KO; ST, MPPs Msi2 WT and KO, respectively. Data as mean +/- SEM. Unpaired Student t test, ****p < 0.0001. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-020-15814-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76831-img0007.jpg)
![Western Blot: BRCC3 Antibody [NBP1-76831] - Analysis of BRCC36 in MCF7 cell lysate with BRCC36 antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Western-Blot-NBP1-76831-img0006.jpg)
![Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat delta K (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76831_rabbit-polyclonal-brcc3-antibody-310202416163777.jpg)
![Western Blot: BRCC3 Antibody - BSA Free [NBP1-76831] - SHMT2 & the BRCC36/BRISC deubiquitinase controls Tat K63Ub & destruction by autophagy.(A) Immunoblot analysis of Tat & GFP protein levels in 2D10 cells depleted of SHMT2 or Cyclin T1, as indicated. TAF4 served as loading control. (B) Immunoblot analysis of Tat protein expression in 2D10 cells depleted of BRCC36 or Cyclin T1. NF-kappa B2 protein levels were monitored to assess any change in T cell signaling. TAF4 served as loading control. (C) Immunoblot analysis of Tat expression in Tet-on-Tat-off HeLa cells depleted of SHMT1, SHMT2 or BRCC36, as indicated above each lane. TAF4 served as loading control. (D) Top, immunoblot analysis of the effects of overexpression of Flag-SHMT1 & FH-BRCC36 proteins on Tat protein levels. TAF4 served as loading control. Bottom, Dual:Luc reporter activity of the HIV-Luc reporter. Plasmids expressing the Flag-vector, Flag-SHMT1 & FH-BRCC36 proteins were tested at 0 ng (blue bar), 20 ng (purple bar), 100 ng (yellow bar) & 500 ng (light blue bar), as indicated. (E) Analysis of FLAG-Tat-101 immunoprecipitates from lysates of BRCC36- or SHMT1- knockdown cells. FLAG-Tat-101 proteins were labelled with HA-tagged ubiquitin, either wild-type Ub (WT), or ubiquitin mutants that selectively support only K63 or K48 ubiquitylation, & Tat proteins were monitored using anti-HA antisera. (F) Immunoblot analysis of the effect of JIB-04 on Tat protein levels in 2D10 cells depleted of SHMT2 or BRCC36. (G) The effect of JIB-04 on wildtype or Tat delta K (+K41) STREP-tagged Tat proteins was shown by immunoblot. TAF4 served as loading control. (H) Model of the role of SHMT2 & BRCC36 in the release of Tat-K63Ub from destruction through chaperone-mediated autophagy or SQSMT1/p62-dependent macroautophagy. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1007071), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76831_rabbit-polyclonal-brcc3-antibody-31020241616070.jpg)
![Immunohistochemistry: BRCC3 Antibody - BSA Free [NBP1-76831] - Immunohistochemistry of BRCC3 in human brain tissue with BRCC3 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76831_rabbit-polyclonal-brcc3-antibody-2711202419313212.jpg)
![Immunocytochemistry/ Immunofluorescence: BRCC3 Antibody - BSA Free [NBP1-76831] - Immunofluorescence of BRCC3 in Human Brain cells with BRCC3 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76831_rabbit-polyclonal-brcc3-antibody-2711202419333441.jpg)
![Western Blot: BRCC3 Antibody (3B1) [NBP1-30429] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat ant imouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: 293T cell lysate Lane 4.: MCF7 cell lysate Lane 5.: NIH3T3 cell lysate.](http://images.novusbio.com/fullsize/BRCC3-Antibody-3B1-Western-Blot-NBP1-30429-img0002.jpg "Western Blot: BRCC3 Antibody (3B1) [NBP1-30429] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BRCC36 (1:1000). Proteins were visualized using a goat ant imouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: HepG2 cell lysate Lane 3.: 293T cell lysate Lane 4.: MCF7 cell lysate Lane 5.: NIH3T3 cell lysate.")
![Immunocytochemistry/Immunofluorescence: BRCC3 Antibody (3B1) [NBP1-30429] - CC/IF analysis of BRCC36 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BRCC36 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/BRCC3-Antibody-3B1-Immunocytochemistry-Immunofluorescence-NBP1-30429-img0006.jpg)
![Immunohistochemistry;BRCC3 Antibody[NBP3-29829]Detection of human BRCC36 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRCC36 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29830_rabbit-brcc3-pab-78202410372622.jpg "Immunohistochemistry;BRCC3 Antibody[NBP3-29829]Detection of human BRCC36 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRCC36 used at a dilution of 1:1,000 . Detection: DAB")
![Western Blot: BRCC3 Antibody [H00079184-B01P] - Analysis of BRCC3 expression in transfected 293T cell line by BRCC3 polyclonal antibody. Lane 1: BRCC3 transfected lysate(34.76 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Western-Blot-H00079184-B01P-img0002.jpg "Western Blot: BRCC3 Antibody [H00079184-B01P] - Analysis of BRCC3 expression in transfected 293T cell line by BRCC3 polyclonal antibody. Lane 1: BRCC3 transfected lysate(34.76 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [H00079184-B01P] - Analysis of purified antibody to BRCC3 on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/BRCC3-Antibody-Immunocytochemistry-Immunofluorescence-H00079184-B01P-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [NBP2-58298] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58298-img0001.jpg "Immunocytochemistry/Immunofluorescence: BRCC3 Antibody [NBP2-58298] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Western Blot: BRCC3 Antibody [NBP2-15609] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/BRCC3-Antibody-Western-Blot-NBP2-15609-img0001.jpg "Western Blot: BRCC3 Antibody [NBP2-15609] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:5000.")