BARD1 Antibody

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Biological Strategies: Western Blot: BARD1 Antibody [NB100-319] - Detection of Human BARD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat, cells. Antibodies: Affinity purified ...read more
Immunoprecipitation: BARD1 Antibody [NB100-319] - Detection of human BARD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

BARD1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 250 and 300 of human BRCA1-associated RING Domain 1 using the numbering given in entry NP_000456.1 (GeneID 580).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BARD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunoprecipitation 2 - 10 ug/mg lysate
Application Notes
*The investigator should determine the optimal working dilution for a specific application.
Publications
Read Publications using NB100-319.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BARD1 Antibody

  • BARD-1
  • BRCA1 associated RING domain 1
  • BRCA1-associated RING domain gene 1
  • BRCA1-associated RING domain protein 1
  • EC 6.3.2.-

Background

BRCA1-Associated RING Domain gene 1 (BARD1) functions as a tumor suppressor, especially in breast and ovarian cancers. It plays a major role in cancer suppression when it forms a heterodimer with BRCA1. It also plays a role in mediating apoptotic stress and p53-dependent apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IP

Publications for BARD1 Antibody (NB100-319)(13)


Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-319 Applications Species
Tembe V, Martino-Echarri E, Marzec KA et al. The BARD1 BRCT domain contributes to p53 binding, cytoplasmic and mitochondrial localization, and apoptotic function. Cell Signal 2015 Jun 3 [PMID: 26022179]
Zhang YQ, Pilyugin M, Kuester D et al. Expression of oncogenic BARD1 isoforms affects colon cancer progression and correlates with clinical outcome. Br J Cancer 2012 Aug [PMID: 22814582]
Chapman JR, Sossick AJ, Boulton SJ et al. BRCA1-associated exclusion of 53BP1 from DNA damage sites underlies temporal control of DNA repair. J Cell Sci 2012 Aug [PMID: 22553214]
Mok MT, Henderson BR. The in vivo dynamic organization of BRCA1-A complex proteins at DNA damage-induced nuclear foci. Traffic 2012 Jun [PMID: 22420687]
Bosse KR, Diskin SJ, Cole KA et al. Common variation at BARD1 results in the expression of an oncogenic isoform that influences neuroblastoma susceptibility and oncogenicity. Cancer Res 2012 Apr [PMID: 22350409]
Wu W, Sato K, Koike A et al. HERC2 is an E3 ligase that targets BRCA1 for degradation. Cancer Res 2010 Aug [PMID: 20631078]
De Nicolo A, Parisini E, Zhong Q et al. Multimodal assessment of protein functional deficiency supports pathogenicity of BRCA1 p.V1688del. Cancer Res 2009 Sep [PMID: 19706752]
Ryser S, Dizin E, Jefford CE et al. Distinct roles of BARD1 isoforms in mitosis: full-length BARD1 mediates Aurora B degradation, cancer-associated BARD1beta scaffolds Aurora B and BRCA2. Cancer Res 2009 Feb [PMID: 19176389]
Nishikawa H, Wu W, Koike A et al. BRCA1-associated protein 1 interferes with BRCA1/BARD1 RING heterodimer activity. Cancer Res 2009 Jan [PMID: 19117993]
De Nicolo A, Tancredi M, Lombardi G et al. A novel breast cancer-associated BRIP1 (FANCJ/BACH1) germ-line mutation impairs protein stability and function. Clin Cancer Res 2008 Jul [PMID: 18628483]
Show All 13 Publications.

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Bioinformatics

Gene Symbol BARD1
Entrez
Uniprot