BRCA1 [p Ser1423] Antibody

Images

 
Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226] - Detection of Human Phospho BRCA1 (S1423) by Western Blot. Samples : Whole cell lysate (50 ug) from HeLa cells treated with 100 uM etoposide for 4 hours (+) or mock ...read more
Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226] - Detection of Brca1 phospho Ser 1423 by Western blot using NB 100-226. Sample whole cell lysate (25 mcg/lane) from HeLa cells treated with 10 Gy IR or untreated. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

BRCA1 [p Ser1423] Antibody Summary

Immunogen
Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human Breast Cancer Gene 1 (GeneID 672) around serine 1423 according to the numbering given in entry NP_009225.1.
Modification
p Ser1423
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BRCA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Flow Cytometry 1:10-1:1000
Application Notes
Use in FLOW cytometry reported in scientific literature ( PMID 24965603)
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-226 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRCA1 [p Ser1423] Antibody

  • BRCA1
  • BRCAI
  • breast and ovarian cancer susceptibility protein 1
  • breast and ovarian cancer sususceptibility protein
  • breast cancer 1, early onset
  • breast cancer type 1 susceptibility protein
  • EC 6.3.2
  • EC 6.3.2.-
  • IRIS
  • PNCA4
  • PSCP
  • subunit 1

Background

BRCA 1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyperphosphorylation during late G1 and S phases of the cell cycle and is transiently dephosphorylated early after M phase. BRCA 1 protein alters in a qualitative and quantitative manner during cell cycle progression. The amount of BRCA protein is highest during S phase and remains elevated toward G2 / M, before it declines in early G1 phase. Inherited loss of BRCA 1 function confers an increased susceptibility for both breast and ovarian cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRCA1 Antibody (NB100-226)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: FLOW, WB.


Filter By Application
FLOW
(1)
WB
(1)
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Filter By Species
Human
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-226 Applications Species
Ntokou A, Klein F, Dontireddy D et al. Characterization of the platelet-derived growth factor receptor-alpha-positive cell lineage during murine late lung development. Am J Physiol Lung Cell Mol Physiol 2015 Nov 1 [PMID: 26320158]
Rosen DB, Leung LY, Louie B et al. Quantitative measurement of alterations in DNA damage repair (DDR) pathways using single cell network profiling (SCNP). J Transl Med 2014 Jul 16 [PMID: 24965603] (FLOW, Human)

Details:
BRCA1 [p Ser1423] antibody used for FLOW on several cell lines - ATM wild type cell lines [U937 and RS4;11 (ATCC), GM00536 and GM09703 (Coriell)], ATM +/- cell line [GM03323 (Coriell)] and two ATM-/- cell lines [GM01526 and GM03189 (Coriell)].
FLOW Human
Rusin M, Zajkowicz A, Butkiewicz D. Resveratrol induces senescence-like growth inhibition of U-2 OS cells associated with the instability of telomeric DNA and upregulation of BRCA1. Mech Ageing Dev 2009 Aug [PMID: 19559722] (WB, Human) WB Human
Cheng W-H, Muftic D, Muftuoglu M et al. WRN Is Required for ATM Activation the S-Phase Checkpoint in Response to Interstr Cross-Link-Induced DNA Double-Str Breaks. Mol Biol Cell;19(9):3923-3933. 2008 [PMID: 18596239]
1 Zaugg, K, Su, Y-W, Reilly, P T, Moolani, Y, Cheung, C C, Hakem, R, Hirao, A, Elledge, S J, Mak, T W. Cross-talk between Chk1 Chk2 in double-mutant thymocytes,. 104, 3805-3810. 2007 [PMID: 17360434]
Nunnari G, Argyris E, Fang J et al. Inhibition of HIV-1 replication by caffeine and caffeine-related methylxanthines. Virology 2005 May [PMID: 15840517]
Andersen JL, Zimmerman ES, DeHart JL et al. ATR and GADD45alpha mediate HIV-1 Vpr-induced apoptosis. Cell Death Differ 2005 Apr [PMID: 15650754]

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Product General Protocols

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Blogs on BRCA1. Showing 1-10 of 20 blog posts - Show all blog posts.

Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily...  Read full blog post.

Further unraveling the role of gamma H2AX in DNA damage response
Our genome experiences a moderate amount of DNA damage in our cells on a daily basis.  This DNA damage can be in response to external environmental factors, or be a result of our internal metabolic processes going awry.  While normal rates of DNA ...  Read full blog post.

The recent relationship of BRCA1 and 53BP1
The p53-binding protein 1 (53BP1) is a DNA damage response factor, which is recruited to nuclear structures at the site of DNA damage.  DNA double-strand breaks (DSBs) are mutations that are detrimental to cell viability and genome stability, and m...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

FANCD2 (Fanconi anemia subunit D2 protein)
Fanconi anemia (FANC) is a rare, autosomal-recessive genetic disorder that is a heterogeneous cancer susceptibility condition that manifests with a wide range of symptoms such as congenital malformations, deteriorating bone marrow failure, DNA-dama...  Read full blog post.

53BP1 - a marker for DNA Double Strand Break
53BP1 (p53 binding protein 1) was originally thought to be an enhancer for p53 transcriptional, but later studies have demonstrated that it is actually a substrate for ataxia telangiectasia mutated (ATM). 53BP1 is a classic late DNA damage response...  Read full blog post.

FANCD2: A big component of the DNA repair crew
The genetic disorder known as Fanconi anemia (FANC) is a heterogeneous, autosomal-recessive cancer susceptibility condition characterized by a wide array of symptoms. These include congenital malformations, progressive bone marrow failure, DNA-damage...  Read full blog post.

BRCA1 - A Critical Tumor Suppressor Gene in Women
Breast cancer 1, early onset (BRCA1) is a well-known tumor suppressor gene that was originally discovered due to its link with early-onset breast and ovarian cancer in women. The BRCA1 protein contains the following domains: RING finger, RAD51-interac...  Read full blog post.

53BP1 - DNA damage is no fun
The 53BP1 (p53 binding protein 1) was initially believed to be a p53 transcriptional enhancing partner, but it has now been established as an ataxia telangiectasia mutated (ATM) substrate. As a late DNA damage response (DDR) marker, 53BP1 appears duri...  Read full blog post.

Breast Cancer Infographic
Breast cancer is caused by malignant cells developing in breast tissue. It is the most commonly diagnosed cancer in women, but advancements in treatment options have seen the death rate decline since the 1990s. Common warning signs of breast cancer in...  Read full blog post.

Showing 1-10 of 20 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol BRCA1
Entrez
Uniprot