BORIS Antibody - BSA Free

Western Blot: BORIS Antibody [NB100-2028] - Analysis of a predominant band corresponding to BORIS in human tissue lysates (arrowhead). Lane 1 contains lysate from human prostate tissue. Lane 2 contains lysate from human spleen tissue. A predominant band at 75 kDa is observed.

Western blot using Affinity Purified anti-BORIS antibody shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead). Lane 1 contains lysate from human prostate tissue. Lane 2 contains lysate from human spleen tissue. A predominant band at ~75 kDa is observed. Molecular weight estimation was made by comparison to prestained MW markers as indicated.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BORIS Antibody - BSA Free Summary

• Description: This affinity-purified antibody is directed against the human BORIS protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
  
  Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified BORIS Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to N-Terminal region near aa 1-30 of human BORIS protein. (Uniprot: Q8NI51)
• Localization: Nuclear and cytoplasmic
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTCFL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:10000
  • Western Blot 1:200-1:2000
• Application Notes: This product has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 75 kDa in size corresponding to BORIS by western blotting in the appropriate cell lysate or extract.
• Control:

  Human Prostate Whole Tissue Lysate (Adult Whole Normal)
  BORIS Overexpression Lysate
  Human Prostate Whole Tissue Lysate (Adult Whole Tumor)

Reactivity Notes

A BLAST analysis was used to suggest cross reactivity with BORIS proteins from human and chimpanzee. Partial reactivity may occur against BORIS from mouse, dog and rat sources based on varying degrees of homology to the immunizing sequence. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BORIS Antibody - BSA Free

• BORISHMGB1L1
• BORIS-like protein
• Brother of the regulator of imprinted sites
• Cancer/testis antigen 27
• CCCTC-binding factor (zinc finger protein)-like
• CCCTC-binding factor
• CT27MGC163358
• CTCF paralog
• CTCF-like protein
• CTCF-T
• dJ579F20.2
• HMG-1L1
• MGC169105
• MGC169106
• putative high mobility group protein 1-like 1
• putative high mobility group protein B1-like 1
• transcriptional repressor CTCFL
• Zinc finger protein CTCF-T

Background

BORIS (Brother of the Regulator of Imprinted Sites) also known as CCTC-binding factor-like protein, is normally only expressed in the testis and expressed in a mutually exclusive manner with CTCF during male germ cell development. However, previous studies have shown that BORIS is abnormally activated in a wide range of human cancers. Expression of BORIS in normally BORIS-negative cells promotes cell growth that may lead to transformation. BORIS maps to the cancer-associated amplification region thought to contain an oncogene or dominant-immortalizing gene. BORIS is a candidate protein for the epigenetic reprogramming factor acting in the male germ line. BORIS is found in both the nucleus and cytoplasm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BORIS Antibody (NB100-2028)(1)

Publication using NB100-2028

• Monk M, Hitchins M, Hawes S. Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS the pluripotency structural gene OCT4, in human preimplantation development. Mol Hum Reprod;14(6):347-355. 2008-01-01 [PMID: 18467432]

Bioinformatics

• Gene Symbol: CTCFL
• Entrez:
  • 140690(Human)
• Uniprot:
  • Q8NI51(Human)