BORIS Overexpression Lysate (Native)

Images

 
Western Blot: BORIS Overexpression Lysate (Adult Normal) [NBL1-09565] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BORIS.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BORIS Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC216042 and based on accession number NM_080618. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens CCCTC-binding factor (zinc finger protein)-like (CTCFL), mRNA.
Gene
CTCFL

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
75.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for BORIS

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BORIS Overexpression Lysate (Native)

  • BORISHMGB1L1
  • BORIS-like protein
  • Brother of the regulator of imprinted sites
  • Cancer/testis antigen 27
  • CCCTC-binding factor (zinc finger protein)-like
  • CCCTC-binding factor
  • CT27MGC163358
  • CTCF paralog
  • CTCF-like protein
  • CTCF-T
  • dJ579F20.2
  • HMG-1L1
  • MGC169105
  • MGC169106
  • putative high mobility group protein 1-like 1
  • putative high mobility group protein B1-like 1
  • transcriptional repressor CTCFL
  • Zinc finger protein CTCF-T

Background

CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for BORIS Lysate (NBL1-09565) (0)

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Bioinformatics

Gene Symbol CTCFL