BMP-4 Antibody (66108) [Biotin]

• Reactivity: Hu, Pm
• Applications: WB, ELISA(Det)
• Clone: 66108
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

BMP-4 Antibody (66108) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BMP‑4
  Ser293-Arg408
  Accession # P12644
• Specificity: Detects human and primate BMP-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human BMP‑2, ‑3, ‑5, ‑6, or ‑7.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMP4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human BMP-4 Antibody (Catalog # MAB7571)
  ELISA Detection: Human BMP-4 Biotinylated Antibody (Catalog # BAM7572)
  Standard: Recombinant Human BMP-4 (Catalog # 314-BP)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-4 Antibody (66108) [Biotin]

• BMP2B
• BMP-2B
• BMP2B1
• BMP2BMCOPS6
• BMP4
• BMP-4
• Bone morphogenetic protein 2B
• bone morphogenetic protein 4
• DVR4
• MCOPS6
• OFC11
• ZYME

Background

BMP-4 is one of at least 20 structurally and functionally related BMPs which are members of the TGF-beta  superfamily. Biologically active BMP-4 is a disulfide-linked homodimer of the carboxy-terminal domain. The morphogenetic effects of BMP-4 on various tissues are mediated by type I and type II BMP receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMP4
• Uniprot:
  • P12644()