BLNK Antibody


Western Blot: BLNK Antibody [NB500-542] - Fig. 1. Western Blotting analysis (reducing conditions) of whole cell lysate of Sp2/0 mouse myeloma cell line using polyclonal anti-mouse BLNK antibody.

Product Details

Reactivity MuSpecies Glossary
Applications WB
1.0 mg/ml

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Available Conjugates
Catalog# & Conjugate Size Price

BLNK Antibody Summary

A fusion protein representing amino acids 171-356 of human BLNK.
The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65).
Sterile filtered
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Sterile filtered PBS (pH 7.4)
15mM Sodium Azide
1.0 mg/ml
Sterile filtered


  • Western Blot 1:1000
Application Notes
Predicted molecular weight 54.7 kDa. Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.

Alternate Names for BLNK Antibody

  • AGM4
  • B cell linker protein
  • BASH
  • B-cell adapter containing a SH2 domain protein
  • B-cell adapter containing a Src homology 2 domain protein
  • B-cell linker protein
  • B-cell linker
  • BLNK-s
  • Cytoplasmic adapter protein
  • LY57
  • MGC111051
  • SLP65
  • SLP-65BLNK-S
  • SLP65Ly57
  • Src homology 2 domain-containing leukocyte protein of 65 kDa


SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa; B cell linker protein), also known as BASH, is an adaptor protein that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syk tyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guanine nucleotide exchange factor, phospholipase Ca2, as well as Grb2 and Nck adaptor proteins; thus represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

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Secondary Antibodies


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Gene Symbol BLNK

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