BLNK Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Biotin

BLNK Antibody [Biotin] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: A fusion protein representing amino acids 171-356 of human BLNK.
• Specificity: The polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BLNK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Predicted molecular weight 54.7 kDa. Western Blot - Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions.
  
  Recommended applications are based on validated applications from the unconjugated base product NB500-542. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS (approx. pH 7.4)
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BLNK Antibody [Biotin]

• AGM4
• B cell linker protein
• BASH
• B-cell adapter containing a SH2 domain protein
• B-cell adapter containing a Src homology 2 domain protein
• B-cell linker protein
• B-cell linker
• BLNK
• BLNK-s
• Cytoplasmic adapter protein
• Ly57
• MGC111051
• SLP65
• SLP-65BLNK-S
• SLP65Ly57
• Src homology 2 domain-containing leukocyte protein of 65 kDa

Background

SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa; B cell linker protein), also known as BASH, is an adaptor protein that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syk tyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guanine nucleotide exchange factor, phospholipase Ca2, as well as Grb2 and Nck adaptor proteins; thus represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BLNK