BLIMP1/PRDM1 Antibody (646702) [Alexa Fluor® 488]

U266 human myeloma cell line was stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC36081G, filled histogram) or isotype control antibody (IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Human peripheral blood mononuclear cells (PBMCs) gated on CD19+CD3-cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC36081G) and Mouse Anti-Human CD38 PerCP-conjugated Monoclonal Antibody (FAB2404C). Quadrant markers were set based on control antibody staining (IC002G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

MM.1S cells were stained with Mouse Anti-Human BLIMP1/PRDM1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC36081G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 646702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

BLIMP1/PRDM1 Antibody (646702) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human BLIMP1/PRDM1
  Lys667-Cys789
  Accession # O75626
• Specificity: Detects human BLIMP1/PRDM1 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRDM1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BLIMP1/PRDM1 Antibody (646702) [Alexa Fluor® 488]

• Beta-interferon gene positive regulatory domain I-binding factor
• beta-interferon gene positive-regulatory domain I binding factor
• BLIMP1
• BLIMP-1
• BLIMP1MGC118925
• blmp-1
• B-lymphocyte-induced maturation protein 1
• MGC118922
• MGC118923
• Positive regulatory domain I-binding factor 1
• PR domain containing 1, with ZNF domain
• PR domain zinc finger protein 1
• PR domain-containing protein 1
• PRDI-BF1MGC118924
• PRDI-binding factor 1
• PRDI-binding factor-1
• PRDM1
• PR-domain zinc finger protein 1
• ZNFPR1A1

Background

Human BLIMP1 (B lymphocyte-induced maturation protein 1, also known as PRDM1) is a 91 kDa zinc-finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789 amino acids (aa) in length, and contains an N-terminal S-E-T domain (aa 64-170) and four C-terminal C2H2-type zinc-finger motifs (aa 539‑645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80 kDa, 691 aa alternate splice form that utilizes an intenal start site. This results in a substitution of three aa for the first 101 aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61‑603 aa in length. Over aa 667-789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BLIMP1/PRDM1 Antibody (IC36081G)(6)

Publications using IC36081G

• Hannah F. Bradford, Liis Haljasmägi, Madhvi Menon, Thomas C.R. McDonnell, Karita Särekannu, Martti Vanker et al. Inactive disease in patients with lupus is linked to autoantibodies to type I interferons that normalize blood IFN alpha and B cell subsets Cell Reports Medicine 1/17/2023 [PMID: 36652906]
• Yijiang Xu, Hang Zhou, Ginell Post, Hong Zan, Paolo Casali Rad52 mediates class-switch DNA recombination to IgD Nature Communications 2/21/2022 [PMID: 35190531]
• Halima A. Shuwa, Tovah N. Shaw, Sean B. Knight, Kelly Wemyss, Flora A. McClure, Laurence Pearmain et al. Alterations in T and B cell function persist in convalescent COVID-19 patients Med (N Y) 6/11/2021 [PMID: 33821250]
• DA Rao, MF Gurish, JL Marshall, K Slowikowsk, CY Fonseka, Y Liu, LT Donlin, LA Henderson, K Wei, F Mizoguchi, NC Teslovich, ME Weinblatt, EM Massarotti, JS Coblyn, SM Helfgott, YC Lee, DJ Todd, VP Bykerk, SM Goodman, AB Pernis, LB Ivashkiv, EW Karlson, PA Nigrovic, A Filer, CD Buckley, JA Lederer, S Raychaudhu, MB Brenner Pathologically expanded peripheral T helper cell subset drives B cells in rheumatoid arthritis Nature, 2017-02-01;542(7639):110-114. 2017-02-01 [PMID: 28150777] (Flow Cytometry, Human)
• Banko Z, Pozsgay J, Szili D, Toth M, Gati T, Nagy G, Rojkovich B, Sarmay G Induction and Differentiation of IL-10-Producing Regulatory B Cells from Healthy Blood Donors and Rheumatoid Arthritis Patients. J Immunol, 2017-01-13;198(4):1512-1520. 2017-01-13 [PMID: 28087671] (Flow Cytometry, Human)
• Kurioka , A, Ussher , J E, Cosgrove , C, Clough , C, Fergusson , J R, Smith , K, Kang , Y-H, Walker , L J, Hansen , T H, Willberg , C B, Klenerman , P MAIT cells are licensed through granzyme exchange to kill bacterially sensitized targets. Mucosal Immunol, 2014-10-01;8(2):429-40. 2014-10-01 [PMID: 25269706] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PRDM1
• Uniprot:
  • O75626()